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![Western Blot: Mitofusin 1 Antibody (3C9) [H00055669-M04] - Analysis of MFN1 expression in HepG2.](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-3C9-Western-Blot-H00055669-M04-img0005.jpg "Western Blot: Mitofusin 1 Antibody (3C9) [H00055669-M04] - Analysis of MFN1 expression in HepG2.")
![Western Blot: Mitofusin 1 Antibody (3C9) [H00055669-M04] - MFN1 monoclonal antibody (M04), clone 3C9 Western Blot analysis of MFN1 expression in HeLa ( Cat # L013V1 ).](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-3C9-Western-Blot-H00055669-M04-img0001.jpg)
![Western Blot: Mitofusin 1 Antibody (3C9) [H00055669-M04] - Western blot analysis of MFN1 over-expressed 293 cell line, cotransfected with MFN1 Validated Chimera RNAi or non-transfected control. Blot probed with H00055669-M04. GAPDH (36.1 kDa) used as loading control.](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-3C9-Western-Blot-H00055669-M04-img0003.jpg)
![Western Blot: Mitofusin 1 Antibody (3C9) [H00055669-M04] - Analysis of MFN1 expression in transfected 293T cell line by MFN1 monoclonal antibody (M04), clone 3C9. Lane 1: MFN1 transfected lysatE (84.2 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-3C9-Western-Blot-H00055669-M04-img0006.jpg)
![ELISA: Mitofusin 1 Antibody (3C9) [H00055669-M04] - Detection limit for recombinant GST tagged MFN1 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-3C9-ELISA-H00055669-M04-img0007.jpg)
![Western Blot: Mitofusin 1 Antibody [NBP1-51841] - Densitometric analyses of protein expression levels relative to the sedentary group with representative image of western blot are shown. n = 5-6/group. Values are expressed by mean +/- SEM expressed as fold change relative to the sedentary group. * P < 0.05, significantly different from the sedentary group. Black bar: fetal hearts from sedentary dams; gray bar: fetal hearts from exercised dams. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC5350185/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-Western-Blot-NBP1-51841-img0010.jpg "Western Blot: Mitofusin 1 Antibody [NBP1-51841] - Densitometric analyses of protein expression levels relative to the sedentary group with representative image of western blot are shown. n = 5-6/group. Values are expressed by mean +/- SEM expressed as fold change relative to the sedentary group. * P < 0.05, significantly different from the sedentary group. Black bar: fetal hearts from sedentary dams; gray bar: fetal hearts from exercised dams. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC5350185/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Mitofusin 1 Antibody [NBP1-51841] - Mfn-1 expressed in mouse neural stem cells. Image from verified customer review.](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-51841-img0008.jpg)
![Immunohistochemistry: Mitofusin 1 Antibody [NBP1-51841] - IHC staining of Mfn1 in mouse intestine.](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-Immunohistochemistry-NBP1-51841-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Mitofusin 1 Antibody [NBP1-51841] - Mitofusin-1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-51841-img0007.jpg)
![Western Blot: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Effect of AIF overexpression on mitochondrial fission and fusion in the cortex. Immunoblotting of OPA1 and Mitofusin 1 in the mitochondrial fraction from cortical tissue of the CL and IL hemispheres at 24 h after HI in WT and AIF Tg mice. The expression of Mitofusin 1 was significantly decreased at 24 h post-HI both in WT and AIF Tg mice, while the short form (82 kDa) and cleavage band of OPA1 were significantly increased at 24 h post-HI in both the WT and AIF Tg mice. Quantification of OPA1 and Mitofusin 1 did not show any significant differences in the CL and IL hemispheres between WT and AIF Tg mice (n=6/group). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2280-z), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-11E9-1H12-Western-Blot-NBP1-71775-img0014.jpg "Western Blot: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Effect of AIF overexpression on mitochondrial fission and fusion in the cortex. Immunoblotting of OPA1 and Mitofusin 1 in the mitochondrial fraction from cortical tissue of the CL and IL hemispheres at 24 h after HI in WT and AIF Tg mice. The expression of Mitofusin 1 was significantly decreased at 24 h post-HI both in WT and AIF Tg mice, while the short form (82 kDa) and cleavage band of OPA1 were significantly increased at 24 h post-HI in both the WT and AIF Tg mice. Quantification of OPA1 and Mitofusin 1 did not show any significant differences in the CL and IL hemispheres between WT and AIF Tg mice (n=6/group). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2280-z), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Antibody was tested in 3T3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-11E9-1H12-Immunocytochemistry-Immunofluorescence-NBP1-71775-img0013.jpg)
![Immunohistochemistry-Paraffin: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Analysis of Mitofusin-1 in mouse liver.](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-11E9-1H12-Immunohistochemistry-Paraffin-NBP1-71775-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Analysis of Mitofusin-1 expression in (1) MEF wild-type and (2) MEF Mitofusin-1-null whole cell lysates using NBP1-71775.](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-11E9-1H12-Western-Blot-NBP1-71775-img0009.jpg)
![Western Blot: Mitofusin 1 Antibody (11E9-1H12) - BSA Free [NBP1-71775] - Effect of AIF overexpression on mitochondrial fission & fusion in the cortex.a Representative immunoblots of mitochondrial fission proteins (P-DRP1 & FIS1) in the mitochondrial fraction from cortical tissue of P9 WT & AIF Tg mice under physiological conditions. Quantification of P-DRP1 & FIS1 did not show a significant difference between WT & AIF Tg mice (n = 6/group). b Representative immunoblots of mitochondrial fusion proteins (OPA1 & MFN1) in the mitochondrial fraction from cortical tissue of P9 WT & AIF Tg mice under physiological conditions. Quantification of OPA1 & MFN1 did not show any significant difference between WT & AIF Tg mice (n = 6/group). c Immunoblotting of P-DRP1and FIS1 in the mitochondrial fraction from the cortical tissue of the CL & IL hemispheres in WT & AIF Tg mice at 24 h after HI. Quantification of P-DRP1 & FIS1 did not show any significant differences in the CL & IL hemispheres between WT & AIF Tg mice (n = 6/group). d Immunoblotting of OPA1 & MFN1 in the mitochondrial fraction from cortical tissue of the CL & IL hemispheres at 24 h after HI in WT & AIF Tg mice. The expression of MFN1 was significantly decreased at 24 h post-HI both in WT & AIF Tg mice, while the short form (82 kDa) & cleavage band of OPA1 were significantly increased at 24 h post-HI in both the WT & AIF Tg mice. Quantification of OPA1 & MFN1 did not show any significant differences in the CL & IL hemispheres between WT & AIF Tg mice (n = 6/group). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32001673), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-71775_mouse-monoclonal-mitofusin-1-antibody-11e9-1h12-310202416163735.jpg)
![Western Blot: Mitofusin 1 Antibody (S05-3A8) [NBP3-19890] - Western blot detection of Mitofusin 1 in CHO-K1, C6 cell lysates using NBP3-19890 (1:1000 diluted). Predicted band size: 84kDa. Observed band size: 84kDa.](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-S05-3A8-Western-Blot-NBP3-19890-img0001.jpg "Western Blot: Mitofusin 1 Antibody (S05-3A8) [NBP3-19890] - Western blot detection of Mitofusin 1 in CHO-K1, C6 cell lysates using NBP3-19890 (1:1000 diluted). Predicted band size: 84kDa. Observed band size: 84kDa.")
![Immunocytochemistry/Immunofluorescence: Mitofusin 1 Antibody [NBP2-57125] - Staining of human cell line U-2 OS shows localization to mitochondria.](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57125-img0001.jpg "Immunocytochemistry/Immunofluorescence: Mitofusin 1 Antibody [NBP2-57125] - Staining of human cell line U-2 OS shows localization to mitochondria.")
![Western Blot: Mitofusin-1 Overexpression Lysate (Adult Normal) [NBL1-13039] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Mitofusin-1.](http://images.novusbio.com/fullsize/Mitofusin-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13039-img0002.jpg "Western Blot: Mitofusin-1 Overexpression Lysate (Adult Normal) [NBL1-13039] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Mitofusin-1.")
![Flow Cytometry: Mitofusin 1 Antibody (11G8) [NBP3-26143] - Overlay Peak curve showing HepG2 cells stained with Mitofusin 1 Antibody (11G8) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26143_rabbit-mitofusin-1-mab-11g8-282202414312714.jpg "Flow Cytometry: Mitofusin 1 Antibody (11G8) [NBP3-26143] - Overlay Peak curve showing HepG2 cells stained with Mitofusin 1 Antibody (11G8) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.")