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![Western Blot: INT4 Antibody [NB100-60660] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. NB100-60660 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.](http://images.novusbio.com/fullsize/INT4-Antibody-Western-Blot-NB100-60660-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: INT4 Antibody [NB100-60660] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. NB100-60660 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: INT4 Antibody [NB100-60660] - Human breast carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).](http://images.novusbio.com/fullsize/INT4-Antibody-Immunohistochemistry-Paraffin-NB100-60660-img0003.jpg)
![Immunohistochemistry-Paraffin: INT4 Antibody [NB100-60660] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-INT4 used at a dilution of 1:250.](http://images.novusbio.com/fullsize/INT4-Antibody-Immunohistochemistry-Paraffin-NB100-60660-img0001.jpg)
![Western Blot: INT4 Antibody [NBP2-16971] - Sample (30 ug of whole cell lysate) A: U87-MG B: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/INT4-Antibody-Western-Blot-NBP2-16971-img0001.jpg "Western Blot: INT4 Antibody [NBP2-16971] - Sample (30 ug of whole cell lysate) A: U87-MG B: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: INT4 Antibody [NBP2-16971] - INT4 antibody detects INTS4 protein at nucleus in mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. INT4 Antibody diluted at 1:500.](http://images.novusbio.com/fullsize/INT4-Antibody-Immunohistochemistry-Paraffin-NBP2-16971-img0004.jpg)
![Immunohistochemistry-Paraffin: INT4 Antibody [NBP2-16971] - Immunohistochemical analysis of paraffin-embedded AGS xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/INT4-Antibody-Immunohistochemistry-Paraffin-NBP2-16971-img0002.jpg)
![Immunohistochemistry-Paraffin: INT4 Antibody [NBP2-16971] - INTS4 antibody detects INTS4 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. INT4 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/INT4-Antibody-Immunohistochemistry-Paraffin-NBP2-16971-img0003.jpg)
![Western Blot: INT4 Antibody [NBP1-83102] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/INT4-Antibody-Western-Blot-NBP1-83102-img0005.jpg "Western Blot: INT4 Antibody [NBP1-83102] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4")
![Immunocytochemistry/Immunofluorescence: INT4 Antibody [NBP1-83102] - Staining of human cell line A-431 shows localization to nucleoli.](http://images.novusbio.com/fullsize/INT4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83102-img0006.jpg)
![Immunohistochemistry-Paraffin: INT4 Antibody [NBP1-83102] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/INT4-Antibody-Immunohistochemistry-Paraffin-NBP1-83102-img0002.jpg)
![Western Blot: INT4 Antibody [NBP3-03410] - Western blot analysis of extracts of various cell lines, using INT4 antibody (NBP3-03410) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/INT4-Antibody-Western-Blot-NBP3-03410-img0002.jpg "Western Blot: INT4 Antibody [NBP3-03410] - Western blot analysis of extracts of various cell lines, using INT4 antibody (NBP3-03410) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: INT4 Antibody [NBP3-03410] - Western blot analysis of extracts of Rat liver, using INT4 antibody (NBP3-03410) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/INT4 Antibody-Western Blot-NBP3-03410-img0003.jpg)
![Immunoprecipitation: INT4 Antibody [NBP3-03410] - Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug INT4 antibody (NBP3-03410). Western blot was performed from the immunoprecipitate using INT4 antibody (NBP3-03410) at a dilution of 1:500.](http://images.novusbio.com/fullsize/INT4-Antibody-Immunoprecipitation-NBP3-03410-img0001.jpg)
![Western Blot: INT4 Antibody [NBP2-87630] - Host: Rabbit. Target Name: INTS4. Sample Type: HepG2 Whole cell lysates. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/INT4-Antibody-Western-Blot-NBP2-87630-img0001.jpg "Western Blot: INT4 Antibody [NBP2-87630] - Host: Rabbit. Target Name: INTS4. Sample Type: HepG2 Whole cell lysates. Antibody Dilution: 1.0ug/ml")
![Western Blot: INT4 Antibody [NBP2-87629] - WB Suggested Anti-INTS4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Hela cell lysate](http://images.novusbio.com/fullsize/INT4-Antibody-Western-Blot-NBP2-87629-img0001.jpg "Western Blot: INT4 Antibody [NBP2-87629] - WB Suggested Anti-INTS4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Hela cell lysate")
![Western Blot: INT4 Overexpression Lysate (Adult Normal) [NBP2-05850] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for INT4.](http://images.novusbio.com/fullsize/INT4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-05850-img0001.jpg "Western Blot: INT4 Overexpression Lysate (Adult Normal) [NBP2-05850] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for INT4.")