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![Western Blot: ATG4B Antibody [NBP2-24709] - Analysis of APG4B in HeLa cell lysate in the 1) absence, and 2) presence of immunizing peptide and 3) RAW cell lysate using NBP2-24709 at 2 ug/ml.](http://images.novusbio.com/fullsize/ATG4B-Antibody-Western-Blot-NBP2-24709-img0001.jpg "Western Blot: ATG4B Antibody [NBP2-24709] - Analysis of APG4B in HeLa cell lysate in the 1) absence, and 2) presence of immunizing peptide and 3) RAW cell lysate using NBP2-24709 at 2 ug/ml.")
![Immunohistochemistry-Paraffin: ATG4B Antibody [NBP2-24709] - Analysis of human heart using NBP2-24709 at 10 ug/ml.](http://images.novusbio.com/fullsize/ATG4B-Antibody-Immunohistochemistry-Paraffin-NBP2-24709-img0002.jpg)
![Western Blot: ATG4B Antibody [NBP2-41307] - Analysis of ATG4B in (A) Ramos and (B) Jurkat cell lysate with ATG4B antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/ATG4B-Antibody-Western-Blot-NBP2-41307-img0003.jpg "Western Blot: ATG4B Antibody [NBP2-41307] - Analysis of ATG4B in (A) Ramos and (B) Jurkat cell lysate with ATG4B antibody at 1 ug/mL.")
![Immunohistochemistry-Paraffin: ATG4B Antibody [NBP2-41307] - Human spleen tissue with ATG4B antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/ATG4B-Antibody-Immunohistochemistry-Paraffin-NBP2-41307-img0001.jpg)
![Immunohistochemistry: ATG4B Antibody [NBP2-41307] - Human spleen tissue with ATG4B antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/ATG4B-Antibody-Immunofluorescence-NBP2-41307-img0002.jpg)
![Western Blot: ATG4B Antibody (9H6M6) [NBP3-16538] - Western blot analysis of extracts of various cell lines, using ATG4B Rabbit mAb (NBP3-16538) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/ATG4B-Antibody-9H6M6-Western-Blot-NBP3-16538-img0002.jpg "Western Blot: ATG4B Antibody (9H6M6) [NBP3-16538] - Western blot analysis of extracts of various cell lines, using ATG4B Rabbit mAb (NBP3-16538) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: ATG4B Antibody (9H6M6) [NBP3-16538] - Immunofluorescence analysis of NIH-3T3 cells using ATG4B Rabbit mAb (NBP3-16538) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ATG4B-Antibody-9H6M6-Immunocytochemistry-Immunofluorescence-NBP3-16538-img0001.jpg)
![Western Blot: ATG4B Antibody [NBP2-57210] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.](http://images.novusbio.com/fullsize/ATG4B-Antibody-Western-Blot-NBP2-57210-img0002.jpg "Western Blot: ATG4B Antibody [NBP2-57210] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.")
![Immunocytochemistry/Immunofluorescence: ATG4B Antibody [NBP2-57210] - Staining of human cell line RT4 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/ATG4B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57210-img0001.jpg)
![Western Blot: ATG4B Antibody (PSH02-53) [NBP3-32058] - Western blot analysis of ATG4B on different lysates with Rabbit anti-ATG4B antibody (NBP3-32058) at 1/2,000 dilution.Lane 1: HeLa cell lysateLane 2: Jurkat cell lysateLane 3: 293T cell lysateLane 4: MCF7 cell lysateLane 5: K-562 cell lysateLane 6: COS-1 cell lysateLane 7: NIH/3T3 cell lysateLane 8: C2C12 cell lysateLane 9: PC-12 cell lysateLane 10: Mouse brain tissue lysateLane 11: Rat brain tissue lysateLane 12: Mouse kidney tissue lysateLane 13: Mouse colon tissue lysateLysates/proteins at 30 µg/Lane.Predicted band size: 44 kDaObserved band size: 47 kDaExposure time: 42 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32058_rabbit-atg4b-mab-psh02-53-3920248453542.jpg "Western Blot: ATG4B Antibody (PSH02-53) [NBP3-32058] - Western blot analysis of ATG4B on different lysates with Rabbit anti-ATG4B antibody (NBP3-32058) at 1/2,000 dilution.Lane 1: HeLa cell lysateLane 2: Jurkat cell lysateLane 3: 293T cell lysateLane 4: MCF7 cell lysateLane 5: K-562 cell lysateLane 6: COS-1 cell lysateLane 7: NIH/3T3 cell lysateLane 8: C2C12 cell lysateLane 9: PC-12 cell lysateLane 10: Mouse brain tissue lysateLane 11: Rat brain tissue lysateLane 12: Mouse kidney tissue lysateLane 13: Mouse colon tissue lysateLysates/proteins at 30 µg/Lane.Predicted band size: 44 kDaObserved band size: 47 kDaExposure time: 42 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: ATG4B Antibody [NBP3-10327] - Immunohistochemical analysis of paraffin-embedded human alveolar cell tissue.](http://images.novusbio.com/fullsize/ATG4B-Antibody-Immunohistochemistry-Paraffin-NBP3-10327-img0001.jpg "Immunohistochemistry-Paraffin: ATG4B Antibody [NBP3-10327] - Immunohistochemical analysis of paraffin-embedded human alveolar cell tissue.")
![Western Blot: ATG4B Antibody [NBP3-10649] - Western blot analysis of ATG4B in Human heart. Antibody dilution at 1 ug/mL](http://images.novusbio.com/fullsize/ATG4B-Antibody-Western-Blot-NBP3-10649-img0001.jpg "Western Blot: ATG4B Antibody [NBP3-10649] - Western blot analysis of ATG4B in Human heart. Antibody dilution at 1 ug/mL")
![Western Blot: ATG4B Antibody (SR1744) [NBP3-21750] - Western blot analysis using NBP3-21750 in Ramos cell lysate.](http://images.novusbio.com/fullsize/nbp3-21750_rabbit-atg4b-mab-sr1744-5720231754886.jpg "Western Blot: ATG4B Antibody (SR1744) [NBP3-21750] - Western blot analysis using NBP3-21750 in Ramos cell lysate.")
![Western Blot: ATG4B Antibody (JE59-97) [NBP3-32057] - Western blot analysis of ATG4B on different lysates with Rabbit anti-ATG4B antibody (NBP3-32057) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: Jurkat cell lysate Lane 3: PC-12 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32057) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32057_rabbit-atg4b-mab-je59-97-278202416285266.jpg "Western Blot: ATG4B Antibody (JE59-97) [NBP3-32057] - Western blot analysis of ATG4B on different lysates with Rabbit anti-ATG4B antibody (NBP3-32057) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: Jurkat cell lysate Lane 3: PC-12 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32057) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Western Blot: ATG4B Overexpression Lysate (Adult Normal) [NBL1-07797] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ATG4B.](http://images.novusbio.com/fullsize/ATG4B-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07797-img0002.jpg "Western Blot: ATG4B Overexpression Lysate (Adult Normal) [NBL1-07797] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ATG4B.")
![Western Blot: ATG4B Overexpression Lysate (Adult Normal) [NBP2-07413] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ATG4B.](http://images.novusbio.com/fullsize/ATG4B-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07413-img0001.jpg "Western Blot: ATG4B Overexpression Lysate (Adult Normal) [NBP2-07413] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ATG4B.")