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![Western Blot: ATG10 Antibody [NBP2-38524] - ATG10 is involved in p62-regulated autophagy. RPE cells were transfected with p62 or scrambled siRNA for 24h and then exposed with 400 H2O2 for 1, 3, 6, 12h respectively. Whole-cells lysates were analyzed by Western blot with anti-p62, ATG10, or beta-actin antibodies. Differences between means were considered statistically significant when p<0.05 by Mann-Whitney U-test. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0171940), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ATG10-Antibody-Western-Blot-NBP2-38524-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: ATG10 Antibody [NBP2-38524] - ATG10 is involved in p62-regulated autophagy. RPE cells were transfected with p62 or scrambled siRNA for 24h and then exposed with 400 H2O2 for 1, 3, 6, 12h respectively. Whole-cells lysates were analyzed by Western blot with anti-p62, ATG10, or beta-actin antibodies. Differences between means were considered statistically significant when p<0.05 by Mann-Whitney U-test. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0171940), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ATG10 Antibody [NBP2-38524] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleus & nucleoli. Antibody staining shown in green.](http://images.novusbio.com/fullsize/ATG10-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38524-img0004.jpg)
![Immunohistochemistry-Paraffin: ATG10 Antibody [NBP2-38524] - Staining of human stomach shows moderate membranous and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATG10-Antibody-Immunohistochemistry-Paraffin-NBP2-38524-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: ATG10 Antibody [NBP2-38524] - ARPE-19 cells were transfected with pooled ATG10 specific siRNAs or scrambled siRNA (30nM) for 24h. ATG10 and ATG5 levels were assessed in protein lysates by Western Blot. (B) RNA isolated from cells was analyzed for ATG10 mRNA expression by qRT- PCR. Differences between means were considered statistically significant when p<0.05 by Mann-Whitney U-test. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28222108/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp2-38524_rabbit-polyclonal-atg10-antibody-20920231412576.jpg)
![Western Blot: ATG10 Antibody [NBP2-38524] - Knockdown of ATG10 makes ARPE-19 cells more sensitive to H2O2.(A) ARPE-19 cells were transfected with pooled ATG10 specific siRNAs or scrambled siRNA (30nM) for 24h. ATG10 & ATG5 levels were assessed in protein lysates by Western Blot. (B) RNA isolated from cells was analyzed for ATG10 mRNA expression by qRT- PCR. Differences between means were considered statistically significant when p<0.05 by Mann-Whitney U-test. (C) Lysates of ARPE-19 cells transfected with siRNAs for 24 hours were assessed for p62 & LC3 levels by Western blot. One set of wells per experiment was exposed to Bafilomycin A1 (75nM) for 3h prior to harvest. beta -actin was used as an internal control. ‘Short’ & ‘Long’ Exposure indicate time of exposure of immunoblot image. (D) 24h after transfection with respective siRNAs, ARPE-19 were subjected to oxidative stress with 800μM H2O2 & incubated at 37°C for 6h. Total cell numbers were determined by trypsinization & counting on TC10® cell counter. (E) Primary fetal RPE cells were transfected with scrambled or pooled ATG10 specific siRNAs & 48 hours after transfection, were subjected to H2O2 for 24 hours & MTT assay was performed. Differences between means were considered statistically significant when p<0.05 by Student’s t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28222108), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-38524_rabbit-polyclonal-atg10-antibody-31020241613645.jpg)
![Western Blot: ATG10 Antibody [NBP2-24594] - Analysis of APG10L in human brain lysate in the 1) absence and 2) presence of immunizing peptide 3) mouse brain and 4) rat brain using NBP2-24594 at 2.0 ug/ml, 6.0 ug/ml and 4.0 ug/ml, respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/ATG10-Antibody-Western-Blot-NBP2-24594-img0004.jpg "Western Blot: ATG10 Antibody [NBP2-24594] - Analysis of APG10L in human brain lysate in the 1) absence and 2) presence of immunizing peptide 3) mouse brain and 4) rat brain using NBP2-24594 at 2.0 ug/ml, 6.0 ug/ml and 4.0 ug/ml, respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: ATG10 Antibody [NBP2-24594] - Atg10 Antibody [NBP2-24594] - Staining of APG10L in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/ATG10-Antibody-Immunohistochemistry-Paraffin-NBP2-24594-img0003.jpg)
![Western Blot: ATG10 Antibody [NBP2-92493] - Analysis of extracts of mouse small intestine, using ATG10 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/ATG10-Antibody-Western-Blot-NBP2-92493-img0001.jpg "Western Blot: ATG10 Antibody [NBP2-92493] - Analysis of extracts of mouse small intestine, using ATG10 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Immunohistochemistry-Paraffin: ATG10 Antibody [NBP2-92493] - Immunohistochemistry of paraffin-embedded Rat testis using ATG10 Rabbit pAb (NBP2-92493) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATG10-Antibody-Immunohistochemistry-Paraffin-NBP2-92493-img0004.jpg)
![Immunohistochemistry-Paraffin: ATG10 Antibody [NBP2-92493] - Immunohistochemistry of paraffin-embedded Human colon using ATG10 Rabbit pAb (NBP2-92493) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATG10-Antibody-Immunohistochemistry-Paraffin-NBP2-92493-img0002.jpg)
![Immunohistochemistry-Paraffin: ATG10 Antibody [NBP2-92493] - Immunohistochemistry of paraffin-embedded Mouse spleen using ATG10 Rabbit pAb (NBP2-92493) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATG10-Antibody-Immunohistochemistry-Paraffin-NBP2-92493-img0003.jpg)
![Western Blot: ATG10 Antibody (2Q3Q3) [NBP3-16649] - Western blot analysis of extracts of various cell lines, using ATG10 Rabbit mAb (NBP3-16649) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/ATG10-Antibody-2Q3Q3-Western-Blot-NBP3-16649-img0002.jpg "Western Blot: ATG10 Antibody (2Q3Q3) [NBP3-16649] - Western blot analysis of extracts of various cell lines, using ATG10 Rabbit mAb (NBP3-16649) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: ATG10 Antibody (2Q3Q3) [NBP3-16649] - Immunofluorescence analysis of NIH-3T3 cells using ATG10 Rabbit mAb (NBP3-16649) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ATG10-Antibody-2Q3Q3-Immunocytochemistry-Immunofluorescence-NBP3-16649-img0001.jpg)
![Western Blot: ATG10 Antibody [NBP3-13109] - Various whole cell extracts (40 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ATG10 antibody (NBP3-13109) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/ATG10-Antibody-Western-Blot-NBP3-13109-img0001.jpg "Western Blot: ATG10 Antibody [NBP3-13109] - Various whole cell extracts (40 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ATG10 antibody (NBP3-13109) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.")
![Immunohistochemistry-Paraffin: ATG10 Antibody [NBP3-13109] - ATG10 antibody detects ATG10 protein at cytoplasm in human ovarian cancer by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. ATG10 antibody (NBP3-13109) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/ATG10-Antibody-Immunohistochemistry-Paraffin-NBP3-13109-img0002.jpg)
![Western Blot: ATG10 Antibody [NBP1-76386] - SK-N-SH cell lysate with ATG10 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.](http://images.novusbio.com/fullsize/ATG10-Antibody-Western-Blot-NBP1-76386-img0002.jpg "Western Blot: ATG10 Antibody [NBP1-76386] - SK-N-SH cell lysate with ATG10 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.")
![Western Blot: Atg10 Overexpression Lysate (Adult Normal) [NBL1-07794] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Atg10.](http://images.novusbio.com/fullsize/Atg10-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07794-img0002.jpg "Western Blot: Atg10 Overexpression Lysate (Adult Normal) [NBL1-07794] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Atg10.")
![Western Blot: Atg10 Overexpression Lysate (Adult Normal) [NBP2-09529] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Atg10.](http://images.novusbio.com/fullsize/Atg10-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09529-img0001.jpg "Western Blot: Atg10 Overexpression Lysate (Adult Normal) [NBP2-09529] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Atg10.")
![Western Blot: ATG10 Antibody (SR1389) [NBP3-21730] - Western blot analysis using NBP3-21730 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21730_rabbit-atg10-mab-sr1389-5720231564719.jpg "Western Blot: ATG10 Antibody (SR1389) [NBP3-21730] - Western blot analysis using NBP3-21730 in HeLa cell lysate.")
![Western Blot: ATG10 Antibody (JE59-86) [NBP3-32055] - Western blot analysis of ATG10 on different lysates with Rabbit anti-ATG10 antibody (NBP3-32055) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: K-562 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 25 kDa Observed band size: 30 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32055) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32055_rabbit-atg10-mab-je59-86-278202415242647.jpg "Western Blot: ATG10 Antibody (JE59-86) [NBP3-32055] - Western blot analysis of ATG10 on different lysates with Rabbit anti-ATG10 antibody (NBP3-32055) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: K-562 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 25 kDa Observed band size: 30 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32055) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![SDS-Page: Atg10 Protein [NBP2-22702]](http://images.novusbio.com/fullsize/Atg10-Protein-SDS-Page-NBP2-22702-img0001.jpg "SDS-Page: Atg10 Protein [NBP2-22702]")