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![Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC2 antibody NB100-373 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-373-img0014.jpg "<b>Biological Strategies Validation. </b>Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC2 antibody NB100-373 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SMC2 Antibody [NB100-373] - SMC2 in HaCaT keratinocytes treated with control shRNA (upper panels) and treated with Nesprin-2-SMC domain specific shRNA (lower panels). Nesprin-2 was detected with mAb K20-478. Bar, 10 um. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/ijcb/2017/8607532/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SMC2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-373-img0015.jpg)
![Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 on HeLa whole cell lysate using NB100-373.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-373-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: SMC2 Antibody [NB100-373] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibod ies : Affinity purified rabbit anti-SMC2 antibody (lot was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-373-img0012.jpg)
![Western Blot: SMC2 Antibody (PSH0-62) [NBP3-32958] - Western blot analysis of SMC2 on different lysates with Rabbit anti-SMC2 antibody (NBP3-32958) at 1/1,000 dilution. Lane 1: HeLa cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: HT-29 cell lysate (30 ug/Lane) Lane 4: HepG2 cell lysate (30 ug/Lane) Lane 5: A431 cell lysate (30 ug/Lane) Lane 6: SH-SY5Y cell lysate (30 ug/Lane) Lane 7: Jurkat cell lysate (30 ug/Lane) Lane 8: Raji cell lysate (30 ug/Lane) Lane 9: COS-1 cell lysate (24 ug/Lane) Predicted band size: 136 kDa Observed band size: 136 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32958) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32958_rabbit-smc2-mab-psh0-62-288202412442988.jpg "Western Blot: SMC2 Antibody (PSH0-62) [NBP3-32958] - Western blot analysis of SMC2 on different lysates with Rabbit anti-SMC2 antibody (NBP3-32958) at 1/1,000 dilution. Lane 1: HeLa cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: HT-29 cell lysate (30 ug/Lane) Lane 4: HepG2 cell lysate (30 ug/Lane) Lane 5: A431 cell lysate (30 ug/Lane) Lane 6: SH-SY5Y cell lysate (30 ug/Lane) Lane 7: Jurkat cell lysate (30 ug/Lane) Lane 8: Raji cell lysate (30 ug/Lane) Lane 9: COS-1 cell lysate (24 ug/Lane) Predicted band size: 136 kDa Observed band size: 136 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32958) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SMC2 Antibody (PSH0-62) [NBP3-32958] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-SMC2 antibody (NBP3-32958) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32958) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32958_rabbit-smc2-mab-psh0-62-29820241420911.jpg)
![Immunocytochemistry/ Immunofluorescence: SMC2 Antibody (PSH0-62) [NBP3-32958] - Immunocytochemistry analysis of HeLa cells labeling SMC2 with Rabbit anti-SMC2 antibody (NBP3-32958) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SMC2 antibody (NBP3-32958) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32958_rabbit-smc2-mab-psh0-62-29202410334030.jpg)
![Western Blot: SMC2 Antibody (PSH0-63) [NBP3-32959] - Western blot analysis of SMC2 on different lysates with Rabbit anti-SMC2 antibody (NBP3-32959) at 1/1,000 dilution. Lane 1: HeLa cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: HT-29 cell lysate (30 ug/Lane) Lane 4: HepG2 cell lysate (30 ug/Lane) Lane 5: A431 cell lysate (30 ug/Lane) Lane 6: SH-SY5Y cell lysate (30 ug/Lane) Lane 7: Jurkat cell lysate (30 ug/Lane) Lane 8: Raji cell lysate (30 ug/Lane) Lane 9: COS-1 cell lysate (24 ug/Lane) Predicted band size: 136 kDa Observed band size: 136 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32959) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32959_rabbit-smc2-mab-psh0-63-288202412493484.jpg "Western Blot: SMC2 Antibody (PSH0-63) [NBP3-32959] - Western blot analysis of SMC2 on different lysates with Rabbit anti-SMC2 antibody (NBP3-32959) at 1/1,000 dilution. Lane 1: HeLa cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: HT-29 cell lysate (30 ug/Lane) Lane 4: HepG2 cell lysate (30 ug/Lane) Lane 5: A431 cell lysate (30 ug/Lane) Lane 6: SH-SY5Y cell lysate (30 ug/Lane) Lane 7: Jurkat cell lysate (30 ug/Lane) Lane 8: Raji cell lysate (30 ug/Lane) Lane 9: COS-1 cell lysate (24 ug/Lane) Predicted band size: 136 kDa Observed band size: 136 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32959) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SMC2 Antibody (PSH0-63) [NBP3-32959] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-SMC2 antibody (NBP3-32959) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32959) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32959_rabbit-smc2-mab-psh0-63-298202414262633.jpg)
![Immunocytochemistry/ Immunofluorescence: SMC2 Antibody (PSH0-63) [NBP3-32959] - Immunocytochemistry analysis of HeLa cells labeling SMC2 with Rabbit anti-SMC2 antibody (NBP3-32959) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SMC2 antibody (NBP3-32959) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32959_rabbit-smc2-mab-psh0-63-29202410334025.jpg)
![Western Blot: SMC2 Antibody [NBP2-49655] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MG](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NBP2-49655-img0003.jpg "Western Blot: SMC2 Antibody [NBP2-49655] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MG")
![Immunocytochemistry/Immunofluorescence: SMC2 Antibody [NBP2-49655] - Immunofluorescent staining of human cell line RH-30 shows localization to nucleus.](http://images.novusbio.com/fullsize/SMC2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49655-img0004.jpg)
![Immunohistochemistry-Paraffin: SMC2 Antibody [NBP2-49655] - Staining of human tonsil shows nuclear positivity in germinal center and non germinal center.](http://images.novusbio.com/fullsize/SMC2-Antibody-Immunohistochemistry-Paraffin-NBP2-49655-img0002.jpg)
![Western Blot: SMC2 Antibody [NB100-371] - Samples : Whole cell lysate (90 ug) from mock transfected (E) or SMC2 transfected (T) HEK293T cells. Antibody : Affinity purified rabbit anti-SMC2 used at 0.1 ug/ml. Detection : Chemiluminescence with a 10 minute exposure.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-371-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: SMC2 Antibody [NB100-371] - Samples : Whole cell lysate (90 ug) from mock transfected (E) or SMC2 transfected (T) HEK293T cells. Antibody : Affinity purified rabbit anti-SMC2 used at 0.1 ug/ml. Detection : Chemiluminescence with a 10 minute exposure.")
![<b>Biological Strategies Validation. </b>Western Blot: SMC2 Antibody [NB100-371] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC2 antibody used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-371-img0003.jpg)
![Western Blot: SMC2 Antibody [NB100-372] - Samples : Whole cell lysate (90 ug) from mock transfected (E) or SMC2 transfected (T) HEK293T cells. Antibody : Affinity purified rabbit anti-SMC2 used at 0.1 ug/ml. Detection : Chemiluminescence with a 10 minute exposure.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-372-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: SMC2 Antibody [NB100-372] - Samples : Whole cell lysate (90 ug) from mock transfected (E) or SMC2 transfected (T) HEK293T cells. Antibody : Affinity purified rabbit anti-SMC2 used at 0.1 ug/ml. Detection : Chemiluminescence with a 10 minute exposure.")
![<b>Biological Strategies Validation. </b>Western Blot: SMC2 Antibody [NB100-372] - Whole cell lysate (90 ug) from mock transfected (E) or SMC2 transfected (T) HEK293T cells. Antibody used at 0.1 ug/ml.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-372-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: SMC2 Antibody [NB100-372] - Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-372-img0003.jpg)
![Western Blot: SMC2 Antibody (GT4312) [NBP3-13601] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMC2 antibody [GT4312] (NBP3-13601) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SMC2-Antibody-GT4312-Western-Blot-NBP3-13601-img0001.jpg "Western Blot: SMC2 Antibody (GT4312) [NBP3-13601] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMC2 antibody [GT4312] (NBP3-13601) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Western Blot: SMC2 Antibody [NBP1-52932] - 293T cells lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NBP1-52932-img0002.jpg "Western Blot: SMC2 Antibody [NBP1-52932] - 293T cells lysate, concentration 0.2-1 ug/ml.")
![Western Blot: SMC2 Antibody (E1M) [NBP2-66582] - HeLa cell lysate.](http://images.novusbio.com/fullsize/SMC2-Antibody-E1M-Western-Blot-NBP2-66582-img0001.jpg "Western Blot: SMC2 Antibody (E1M) [NBP2-66582] - HeLa cell lysate.")