![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human testis.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-85937-img0015.jpg "Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human testis.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: MPSASASRKSQEKPREIMDAAEDYAKERYGISSMIQSQEKPDRVLVRVRDLTIQKADEVVWVRARVHTSRAKGKQCFLVLRQQQFNVQALVAVGDHASKQMVKFAANINKESIVDVEGVVRKVNQKIGSCTQQDV |
| Predicted Species | Rat (94%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DARS1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Publications using NBP1-85937 | Applications | Species |
|---|---|---|
| Frohlich D, Suchowerska AK, Spencer ZH et al. In vivocharacterization of the aspartyl-tRNA synthetase DARS: Homing in on the leukodystrophy HBSL. Neurobiol. Dis. 2017-01-01 [PMID: 27816769] (Mouse) | Mouse | |
| Klugmann M, Suchowerska A, Housley G, Fröhlich D Histological and biochemical methods to assess aminoacyl-tRNA synthetase expression in human post-mortem brain tissue Rare Disease and Orphan Drugs Journal 2023-04-20 (Western Blot, Human) | Western Blot | Human |
| Frohlich D, Suchowerska AK, Voss C et al. Expression Pattern of the Aspartyl-tRNA Synthetase DARS in the Human Brain. Front Mol Neurosci 2018-03-20 [PMID: 29615866] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for DARS Antibody (NBP1-85937)Find related products by research area.
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| Gene Symbol | DARS1 |
![Western Blot: DARS Antibody [NBP1-85937] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/DARS-Antibody-Western-Blot-NBP1-85937-img0007.jpg "Western Blot: DARS Antibody [NBP1-85937] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Western Blot: DARS Antibody [NBP1-85937] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-DARS antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/DARS-Antibody-Western-Blot-NBP1-85937-img0010.jpg "Western Blot: DARS Antibody [NBP1-85937] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-DARS antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human cerebral cortex, colon, kidney and testis using Anti-DARS antibody NBP1-85937 (A) shows similar protein distribution across tissues to independent antibody NBP1-86027 (B).](http://images.novusbio.com/fullsize/DARS-Antibody-Western-Blot-NBP1-85937-img0011.jpg "Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human cerebral cortex, colon, kidney and testis using Anti-DARS antibody NBP1-85937 (A) shows similar protein distribution across tissues to independent antibody NBP1-86027 (B).")
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-85937-img0013.jpg "Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human cerebral cortex.")
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human kidney.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-85937-img0014.jpg "Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human kidney.")
![Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human colon.](http://images.novusbio.com/fullsize/DARS-Antibody-Immunohistochemistry-Paraffin-NBP1-85937-img0016.jpg "Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human colon.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")