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DARS Antibody

Images

 
Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human testis.
Western Blot: DARS Antibody [NBP1-85937] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Genetic Strategies: Western Blot: DARS Antibody [NBP1-85937] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-DARS antibody. Remaining relative intensity is ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human cerebral cortex, colon, kidney and testis using Anti-DARS antibody NBP1-85937 (A) shows similar protein ...read more
Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human cerebral cortex.
Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human kidney.
Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human colon.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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DARS Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MPSASASRKSQEKPREIMDAAEDYAKERYGISSMIQSQEKPDRVLVRVRDLTIQKADEVVWVRARVHTSRAKGKQCFLVLRQQQFNVQALVAVGDHASKQMVKFAANINKESIVDVEGVVRKVNQKIGSCTQQDV
Predicted Species
Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DARS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
DARS Protein (NBP1-85937PEP)
Publications
Read Publications using
NBP1-85937 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DARS Antibody

  • aspartate tRNA ligase 1, cytoplasmic
  • Aspartate--tRNA ligase
  • aspartyl-tRNA synthetase
  • aspartyl-tRNA synthetase, cytoplasmic
  • aspRS
  • Cell proliferation-inducing gene 40 protein
  • cell proliferation-inducing protein 40
  • DKFZp781B11202
  • EC 6.1.1.12
  • MGC111579

Background

Aspartyl-tRNA synthetase (DARS) is part of a multienzyme complex of aminoacyl-tRNA synthetases. Aspartyl-tRNA synthetase charges its cognate tRNA with aspartate during protein biosynthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DARS Antibody (NBP1-85937)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: WB, Western Blot.


Filter By Application
WB
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
All Species
Showing Publications 1 - 3 of 3.
Publications using NBP1-85937 Applications Species
Frohlich D, Suchowerska AK, Spencer ZH et al. In vivocharacterization of the aspartyl-tRNA synthetase DARS: Homing in on the leukodystrophy HBSL. Neurobiol. Dis. 2017-01-01 [PMID: 27816769] (Mouse) Mouse
Klugmann M, Suchowerska A, Housley G, Fröhlich D Histological and biochemical methods to assess aminoacyl-tRNA synthetase expression in human post-mortem brain tissue Rare Disease and Orphan Drugs Journal 2023-04-20 (Western Blot, Human) Western Blot Human
Frohlich D, Suchowerska AK, Voss C et al. Expression Pattern of the Aspartyl-tRNA Synthetase DARS in the Human Brain. Front Mol Neurosci 2018-03-20 [PMID: 29615866] (WB, Human) WB Human

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Research Areas for DARS Antibody (NBP1-85937)

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Blogs on DARS

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Bioinformatics

Gene Symbol DARS1