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Apolipoprotein E/ApoE Antibody (1H4)

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Western Blot: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110] - Analysis using ApoE mAb against HEK293 (1) and ApoE (AA: 20-267)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110] - Analysis using ApoE mAb against HEK293 (1) and ApoE (AA: 20-267)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110] - Analysis of paraffin-embedded liver cancer tissues (left) and brain tissues (right) using ApoE mouse mAb with DAB staining.
Flow Cytometry: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110] - Analysis of HepG2 cells using ApoE mouse mAb (green) and negative control (purple).
ELISA: Apolipoprotein E/ApoE Antibody (1H4) [NBP2-22110] - Red: Control Antigen (100 ng); Purple: Antigen (10 ng); Green: Antigen (50 ng); Blue: Antigen (100 ng).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC
Clone
1H4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Apolipoprotein E/ApoE Antibody (1H4) Summary

Immunogen
Purified recombinant fragment of human ApoE expressed in E. coli. [UniProt# P02649]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
APOE
Purity
Unpurified
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Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Bladder Membrane Tissue Lysate (Adult Membrane Normal)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for Apolipoprotein E/ApoE Antibody (1H4)

  • APOE
  • Apo-E
  • Apolipoprotein E
  • apolipoprotein E3
  • late onset)
  • MGC1571

Background

Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Tissue specificity: Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Apolipoprotein E/ApoE Antibody (NBP2-22110). (Showing 1 - 1 of 1 FAQ).

  1. Was the Flow Cytometry image which appears at the datasheet done on the HEPG2 cells by Intracellular staining with or without Golgi stop reagent?
    • It was intracellular staining.  We permeabilize the cells with 0.1% Triton x100 added for 15 min. Therefore, we anticipate that the "Golgi stop reagent" was not used, and since enough Apolipoprotein E was trapped in the cytoplasmic vesicles, like Golgi apparatus, at the time of fixation, and thus that reagent was not required to detect the protein target.

Control Lysate(s)

Human Bladder Membrane Tissue Lysate (Adult Membrane Normal)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol APOE
Entrez
Uniprot