LC3 antibody

LC3 is distributed ubiquitously in eukaryotes and is a heavily studied autophagy biomarker that was originally identified as a subunit of MAP1A and MAP1B. Because autophagy is a crucial process for maintaining normal neural networks and function, understanding neuronal autophagy is important. Young's group at Univ.

Microtubule-associated protein light chain 3 (LC3) is considered one of the definitive markers of autophagy, and its use is widespread in labs throughout the world. Despite its popularity, there are several considerations when employing LC3 antibodies in immunoassays, Western blots in particular.

MAP1LC3 (shortened to LC3 in our antibody catalog) is one of four mammalian homologues of autophagy-related protein 8 (Atg8). It has been identified as a light chain subunit of the microtubule-associated proteins MAP1A/MAP1B. A modified form of LC3, LC3-II, has been identified as a marker for the autophagosomal membrane in mammals.

In rodents, MAP1LC3 (Microtubule-associated protein 1 light chain 3) is expressed in the renal visceral epithelial cells, or podocytes. LC3 antibody analysis has shown the protein accumulates in its membrane-bound form, LC3II, following conversion from LC3I.