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Autophagy

Best genes to knockout to control autophagic flux

Autophagy interactive signaling pathway, Novus Biologicals, macroautophagy induction, autophagosome maturation, microautophagy, autolysosome, elongation

By Christina Towers, PhD

Tags: 
Autophagy
Autophagic flux
LC3
LC3B
LC3-II
Chloroquine
Bafilomycin
Autophagy Inhibitors
ATGs
ATG5
ATG7
Atg3
ATG12
stx17
p62-sqstm1
ATG13
Apoptosis
Christina Towers
autophagosome
Autophagy flux

Mechanisms of Neurodegeneration: Mitochondrial Dysfunction and Oxidative Stress

3D image of dopaminergic neurons shown in green, Bio-Techne.

By Michalina Hanzel, PhD

Tags: 
Alzheimer’s
Parkinson’s
Huntington’s
Autophagy
Alpha-Synuclein
ndufa1
Dopamine
Dopamine D1 R/DRD1
Oxidative Stress
Reactive Oxygen Species
Michalina Hanzel

Make each cell count: How to assess autophagy using flow cytometry

LC3B expression in HeLa cells treated with chloroquine overnight, ICC. Novus Biologicals

Kristy R. Howell, PhD

Tags: 
Autophagy
autophagosome
LC3
LC3B
alpha Tubulin
GFP
mCherry
Autophagy flux
Chloroquine
rapamycin
Flow cytometry
Kristy Howell
Jurkat cell
MIFC
puncta
fluorescence
Immunocytochemistry
Immunofluorescence

Mechanisms of Neurodegeneration: Protein aggregation and failure of autophagy

Alzheimer’s disease video Novus Biologicals

By Michalina Hanzel, PhD

Tags: 
Alzheimer’s
Parkinson’s
Huntington’s
beta-amyloid
Presenilin-1
macroautophagy
Autophagy
ATG9A
mTOR
Huntingtin
Alpha-Synuclein
beclin-1
APP
Michalina Hanzel

How to visualize autophagy by microscopy

LC3B and alpha tubulin expression in HeLa cells treated with Chloroquine, ICC, immunocytochemistry, immunofluorescence

By Christina Towers, PhD

Tags: 
Autophagy
autophagosome
LC3
LC3B
p62/SQSTM1
alpha Tubulin
GFP
Mitophagy
mCherry
Autophagic flux
Chloroquine
Christina Towers
Autophagy flux
microscopy

The LC3 A, B, C’s and 1, 2, 3’s

Atg5 expression in HeLa cells, ICC, immunocytochemistry, immunofluorescence

By Christina Towers, PhD

Tags: 
Autophagy flux
LC3
LC3B
LC3-II
Autophagy
Autophagy Inhibitors
ATG4
ATG5
ATG12
LC3A
LC3C
GABARAP
GABARAPL1
GABARAPL2
Chloroquine
Bafilomycin-A1
ATG7
ATG8
Christina Towers
autophagosome

Best way to quantitatively measure Autophagic Flux

LC3B expression in HeLa cells, ICC, immunocytochemistry, immunofluorescence

By Christina Towers, PhD

Tags: 
Autophagy flux
LC3
LC3B
LC3-II
Chloroquine
Bafilomycin
Autophagy
Autophagy Inhibitors
Christina Towers
autophagosome
GFP
Flow cytometry
ratiometric
RFP
mCherry

Animal Models to Study Autophagy

Autophagy Signaling Pathway, Novus Biologicals, macroautophagy induction, elongation, microautophagy, autophagosome maturation, autolysosome

By Christina Towers, PhD

Tags: 
ULK1
ATG13
rb1cc11
beclin-1
AMBRA1
ATG14
vps14/pik3r4
VPS34
ATG9A
ATG7
Atg10
ATG12
ATG5
ATG4B
Atg3
LC3B
p62-sqstm1
Autophagy
autophagosome
Christina Towers
mCherry
GFP
Zebrafish
Animal models

Best Methods to Induce and Inhibit Autophagy Pharmacologically

VPS35 expression in endosomes and lysosomes of human U-2 OS cells, ICC

By Christina Towers, PhD

Tags: 
Autophagy
autophagosome
LC3
LC3B
Bafilomycin
Chloroquine
Lysosome
Western blot
Beclin 1
VPS34
ULK1
ATG4B
mTOR
autophagy induction
autophagy inhibition
Christina Towers
Cancer
rapamycin
Autophagic flux

RNA-binding protein Staufen1 conspires with Atxn2 in stress granules to cause neurodegeneration by dysregulating RNA metabolism

PCP4 expression in mouse cerebellum Purkinje neurons, IHC

By Jamshed Arslan Pharm.D.

Tags: 
Neuroscience
Purkinje neurons
Staufen1
ATXN2
SCA2
PolyQ
Aldolase C
Pcp2
Calb1
ALS
neurodegeneration
motor deficit
Pcp4
Rgs8
Fam107b
Homer3
LC3
p62/SQSTM1
Autophagy
movement disorder

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