Antibodies

Anti-AGE antibodies are not the elixir of youth as their name might imply! They have nevertheless provided new information about Alzheimer’s disease, the major cause of dementia in old age.

Extra-bright antibodies increase the specificity and sensitivity of any fluorescence-based procedure. They are stable over a wide pH range and are also highly ‘photo stable.’

Novus Biologicals now offer more than 1,150 antibodies conjugated to DyLight® fluorescent dyes.

As fluorescent imaging technology advances, there is a growing drive to achieve increasingly quantitative measurement of fluorescent signals.

Immunohistochemical testing using antibody panels has a valuable role in cancer diagnosis. Some tumors, especially more malignant ones, tend to lose the appearance of the tissue of origin. However, knowing the tissue of origin assists the physician in selecting the best treatment thus a differential diagnosis is required.

Western blotting combines gel electrophoresis with use of a membrane to separate and identify target proteins using antibodies. Proteins are separated into bands using electrophoresis, and are then transferred to a membrane using filter-paper capillary action or an electroblotting technique. The effectiveness of the transfer can be checked by means of a stain – typically Ponceau S.

Western blot is a widely used immunoassay technique, used to identify proteins. Many people enter Western blot research without having a clear idea of how the technique relates to antibody usage, so we at Novus Biologicals thought it would be interesting to give a general review of this subject.

Human stem cell research has proved a controversial topic, but the latest developments could alter the debate significantly.

Recombinant proteins are those derived from recombinant DNA. Recombinant DNA is an artificial form of DNA, made by combining protein sequences which would not occur in their natural state.

Antibody conjugation involves tagging on a protein, compound or dye, in order to track its interaction with specific antigens. It is a routine procedure in immunoassays. We at Novus Biologicals have a vast number of products in our conjugated antibody catalog, to which we are constantly adding.

A conjugated antibody (also known as a tagged, loaded or labeled antibody) is one that has been attached to a substrate such as an enzyme, toxin or inorganic compound. Modern immunoassay techniques frequently use proteins conjugated with a fluorescent dye. Contemporary antibody sources sometimes supply a wide range of both polyclonal and monoclonal conjugated antibodies.

Bcl-2 antibodies are used as markers for the Bcl-2 gene. Bcl-2 suppresses apoptosis (controlled cell death) by inhibiting caspase activity and controlling cell membrane permeability. It does this by forming mitochondrial pores on the outer membrane of the mitochondria. However, its exact mechanism of action is still not completely understood.