Histone modification is known to affect transcriptional access to chromatin. Therefore, high quality histone modification specific antibodies are necessary to understand and explain the specific roles that these epigenetic modifications play in transcription regulation. Unfortunately, many of the commercially available histone modification antibodies are designed against short immunizing peptides and lack specificity to the full-length modified histone.
In work presented at last week’s meeting of the Amercian Society for Cell Biology, researchers at 21st Century Biochemicals and Novus Biologicals developed a highly specific EpiPlusTM antibody line. These antibodies were then characterized in Western blot, ChIP and immunostain on full-length forms of various epigenetically modified Histone H3 and H4 to ensure specificity. ChIP testing was performed using Novus' ChromataChIP kits. This thorough validation in various applications on full-length proteins ensures that the EpiPlusTM line is the best histone modification specific antibodies available on the market.
Source:
Fishman J, Homon A, Lamsa E, et al. The Complete Chemical Synthesis of Histones H3 and H4 Containing Epigenetic Modifications and Their Use in Characterizing Arginine Methylated Histone Antibodies. Poster presented at ASCB. 21st Century Biochemicals, Marlborough, MA 01752, Novus Biologicals, LLC, 8100 Southpark Way, Littleton, CO 80120
Comments
The chromata chIP kit is excellent! It is efficient, the protocol is very easy, and it works. The kit was very easy to follow.