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XPB Antibody [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, Mu, Rb, V-Vi, Rt, Bv, Pm-OrSpecies Glossary
Applications WB, IP, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-61060AF350
Alexa Fluor 405
NB100-61060AF405
Alexa Fluor 488
NB100-61060AF488
Alexa Fluor 532
NB100-61060AF532
Alexa Fluor 594
NB100-61060AF594
Alexa Fluor 647
NB100-61060AF647
Alexa Fluor 700
NB100-61060AF700
Alexa Fluor 750
NB100-61060AF750
Allophycocyanin
NB100-61060APC
Biotin
NB100-61060B
DyLight 350
NB100-61060UV
DyLight 405
NB100-61060V
DyLight 488
NB100-61060G
DyLight 550
NB100-61060R
DyLight 594
NB100-61060DL594
DyLight 650
NB100-61060C
DyLight 680
NB100-61060FR
DyLight 755
NB100-61060IR
HRP
NB100-61060H
Janelia Fluor 525
NB100-61060JF525
Janelia Fluor 549
NB100-61060JF549
Janelia Fluor 646
NB100-61060JF646
Janelia Fluor 669
NB100-61060JF669
PE
NB100-61060PE
PerCP
NB100-61060PCP
Unconjugated
NB100-61060
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XPB Antibody [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
The immunogen recognized by this antibody maps to a region between residue 732 and 782 of human excision repair cross-complementing rodent repair deficiency, complementation group 3 using the numbering given in entry NP_000113.1 (GeneID 2071).
Predicted Species
Rat (100%), Bovine (100%), Orangutan (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ERCC3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Use in Epstein-Barr virus reported in scientific publication (PMID: 32434920). Rabbit reactivity reported from CiteAb.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for XPB Antibody [CoraFluor™ 1]

  • Basic transcription factor 2 89 kDa subunit
  • BTF2 p89
  • BTF2
  • DNA excision repair protein ERCC-3
  • DNA repair protein complementing XP-B cells
  • EC 3.6.1
  • EC 3.6.4.12
  • ERCC3
  • excision repair cross-complementing rodent repair deficiency, complementationgroup 3 (xeroderma pigmentosum group B complementing)
  • GTF2H
  • RAD25
  • TFIIH basal transcription factor complex 89 kDa subunit
  • TFIIH basal transcription factor complex helicase XPB subunit
  • TFIIH p89
  • TFIIH
  • Xeroderma pigmentosum group B-complementing protein
  • xeroderma pigmentosum, complementation group B
  • XPB
  • XPBC
  • XPBTFIIH 89 kDa subunit

Background

ERCC3 is a component of the core TFIIH basal transcription factor and functions as an ATP-dependent 3'-5' DNA helicase. ERCC3 is involved in nucleotide excision repair and is the cause of xeroderma pigmentosum complementation group B (XPB), also known as xeroderma pigmentosum II (XP2). Additionally, it has been found to be the cuase of Cockayne syndrome, and trichothiodystrophy (TTD). XPB is an autosomal recessive disease characterized by skin photosensitivity and a predisposition to skin cancer, and neurological abnormalities. Cockayne syndrome and TTD are similarly characterized by photosensitivity and neural abnormalities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ERCC3