WNK4 Antibody - BSA Free

WNK4 (protein kinase with no lysine 4) is a novel serine/threonine kinase which plays critical role in regulation of electrolyte homeostasis, signal transduction, survival and proliferation. WNK4 is highly expressed in kidney, colon and skin where it forms a part of tight junction complex and interacts with/activate several proteins including SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5, NEDD4L and TRPV6. It control SLC12A3 (thiazide-sensitive Na-Cl cotransporter) activity by phosphorylation process that prevents membrane trafficking of SLC12A3. WNK4 inhibits KCNJ1 (renal K+ channel) via a kinase-independent action leading to induction of protein clearance from the cell surface by clathrin-dependent endocytosis. WNK4 acts as a molecular switch that controls NaCl reabsorption and K+ secretion for maintaining integrated homeostasis. Autophosphorylation of Ser-335 is essential for WNK4's activity whereas phosphorylation of Ser-331 also increase its activity. Mutations of WNK4 can cause pseudohypoaldosteronism type alpha (PHA alpha), an autosomal dominant disease characterized by hyperkalemia, metabolic acidosis and hypertension.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: EM, IF/IHC, IHC-Fr, IHC-P, IP, WB.

Showing Publications 1 - 10 of 14. Show All 14 Publications. Collapse Publications.

Publications using NB600-284	Applications	Species
Yang HJ, Kim MJ, Kim SS, Cho YW Melatonin modulates nitric oxide-regulated WNK-SPAK/OSR1-NKCC1 signaling in dorsal raphe nucleus of rats The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2021-09-01 [PMID: 34448462] (WB, Rat)	WB	Rat
Zhang Y, Ye L, Yuan H, Duan D CFTR plays an important role in the regulation of vascular resistance and high-fructose/salt-diet induced hypertension in mice Journal of Cystic Fibrosis 2020-12-01 [PMID: 33279469] (WB, Mouse)	WB	Mouse
Frame AA, Puleo F, Kim K et al. Sympathetic regulation of the NCC in norepinephrine-evoked salt-sensitive hypertension in Sprague-Dawley rats Am. J. Physiol. Renal Physiol. 2019-10-14 [PMID: 31608673] (Rat)		Rat
Kim MJ, Yang HJ, Kim Y et al. Role of nitric oxide and WNK-SPAK/OSR1-KCC2 signaling in daily changes in GABAergic inhibition in the rat dorsal raphe neurons. Neuropharmacology. 2018-03-27 [PMID: 29596900] (Rat)		Rat
Huguet L, Le Dudal M, Livrozet M et al. High frequency and wide range of human kidney papillary crystalline plugs. Urolithiasis 2017-12-12 [PMID: 29234857] (IF/IHC, Human)	IF/IHC	Human
Li J, Hatano R, Xu S et al. Gender difference in kidney electrolyte transport. I. Role of AT1a receptor in thiazide-sensitive Na+-Cl-cotransporter activity and expression in male and female mice. Am. J. Physiol. Renal Physiol. 2017-08-01 [PMID: 28566500] (WB, Mouse)	WB	Mouse
Bhuiyan M, Song S, Yuan H et al. WNK-Cab39-NKCC1 signaling increases the susceptibility to ischemic brain damage in hypertensive rats. J Cereb Blood Flow Metab 2017-01-01 [PMID: 27798271] (WB, Rat)	WB	Rat
Roy A, Goodman J H et al. Generation of WNK1 knockout cell lines by CRISPR/Cas-mediated genome editing. Am J Physiol Renal Physiol 2015-02-15 [PMID: 25477473] (WB, Human)	WB	Human
Andrukhova O, Smorodchenko A, Egerbacher M et al. FGF23 promotes renal calcium reabsorption through the TRPV5 channel. EMBO J. 2014-02-03 [PMID: 24434184] (IP, IHC-P, EM, WB, Mouse)	IP, IHC-P, EM, WB	Mouse
Ohno M, Uchida K, Ohashi T et al. Immunolocalization of WNK4 in mouse kidney. Histochem Cell Biol. 2011-06-10 [PMID: 21660484] (WB, Mouse)	WB	Mouse
Uchida S. Pathophysiological roles of WNK kinases in the kidney Pflugers Arch 2010-09-01 [PMID: 20490538] (IF/IHC, Mouse)	IF/IHC	Mouse
Subramanya AR, Liu J, Ellison DH et al. WNK4 Diverts the Thiazide-sensitive NaCl Cotransporter to the Lysosome Stimulates AP-3 Interaction. J Biol Chem;284(27):18471-18480. 2009-01-01 [PMID: 19401467] (WB, Mouse, Human)	WB	Mouse, Human
Song, J et al. Regulation of blood pressure, the epithelial sodium channel (ENaC), and other key renal sodium transporters by chronic insulin infusion in rats. Am J Physiol Renal Physiol;290(5):F1055-64. 2006-05-01 [PMID: 16303859] (WB, Rat)	WB	Rat
Kahle, KT et al. WNK4 regulates apical and basolateral Cl- flux in extrarenal epithelia. Proc Natl Acad Sci U S A;101(7):2064-9. 2004-02-17 [PMID: 14769928] (WB, IHC-Fr, Human, Mouse)	WB, IHC-Fr	Human, Mouse
Show All 14 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	4 reviewed by: Verified Customer	WB	Rat	12/13/2017	View Summary Application Western Blot Sample Tested Brain (cerebral cortex) Species Rat Lot B-2

Array NB600-284

• WNK4 Antibodies
• WNK4 Lysate
• WNK4 Proteins