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WIPI2 Antibody

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Western Blot: WIPI2 Antibody [NBP3-13110] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with WIPI2 antibody (NBP3-13110) diluted at 1:2000. The HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: WIPI2 Antibody [NBP3-13110] - WIPI2 antibody detects WIPI2 protein at Preautophagosomal structure membrane by immunofluorescent analysis. Sample: A431 cells were fixed in ...read more
Immunohistochemistry-Paraffin: WIPI2 Antibody [NBP3-13110] - WIPI2 antibody detects WIPI2 protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. WIPI2 antibody ...read more
Western Blot: WIPI2 Antibody [NBP3-13110] - Various whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with WIPI2 antibody (NBP3-13110) diluted at 1:2000. The HRP-conjugated ...read more

Product Details

Summary
Reactivity Hu, Rt, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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WIPI2 Antibody Summary

Description
Centrifuge briefly prior to opening.
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human WIPI2. The exact sequence is proprietary.
Predicted Species
Mouse (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WIPI2
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for WIPI2 Antibody

  • ATG18B
  • Atg21
  • CGI-50
  • DKFZP434J154
  • DKFZp686P02188
  • FLJ12979
  • FLJ14217
  • FLJ42984
  • WD repeat domain phosphoinositide-interacting protein 2
  • WD repeat domain, phosphoinositide interacting 2
  • WD40 repeat protein interacting with phosphoinositides 2
  • WIPI-2
  • WIPI49-like protein 2

Background

WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for WIPI2 Antibody (NBP3-13110) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol WIPI2