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VPS29 Antibody

Images

 
Western Blot: VPS29 Antibody [NBP2-20871] - Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE gel, diluted at 1:2000.
Immunohistochemistry-Paraffin: VPS29 Antibody [NBP2-20871] - Human breast cancer, using VPS29 antibody at 1:250 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15 min.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, Xp, ZeSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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VPS29 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human VPS29. The exact sequence is proprietary.
Localization
Cytoplasm; Membrane; Peripheral membrane protein; Endosome membrane; Peripheral membrane protein
Predicted Species
Mouse (98%), Rat (97%), Chicken (96%), Canine (100%), Xenopus (95%), Zebrafish (94%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VPS29
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (95%), Drosophila (80%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for VPS29 Antibody

  • DC15
  • DC7
  • DKFZp564F0223
  • EC 3.1.3.3
  • FLJ20492
  • hVPS29
  • PEP11 homolog
  • PEP11
  • retromer protein
  • vacuolar protein sorting 29 (yeast homolog)
  • vacuolar protein sorting 29 (yeast)
  • vacuolar protein sorting 29 homolog (S. cerevisiae)
  • vacuolar protein sorting-associated protein 29
  • vacuolar sorting protein VPS29/PEP11
  • Vesicle protein sorting 29
  • x 007 protein

Background

This gene belongs to a group of vacuolar protein sorting (VPS) genes that, when functionally impaired, disrupt the efficient delivery of vacuolar hydrolases. The protein encoded by this gene is a component of a large multimeric complex, termed the retromer complex, which is involved in retrograde transport of proteins from endosomes to the trans-Golgi network. This VPS protein may be involved in the formation of the inner shell of the retromer coat for retrograde vesicles leaving the prevacuolar compartment. Alternative splice variants encoding different isoforms, and usage of multiple polyadenylation sites have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol VPS29
Uniprot