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VGLUT1 Antibody (CL2754)

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Orthogonal Strategies: Immunohistochemistry-Paraffin: VGLUT1 Antibody (CL2754) [NBP2-46627] - VGLUT1 Antibody (2754) [NBP2-46627] - Staining in human cerebral cortex and tonsil tissues. Corresponding VGLUT1 ...read more
Western Blot: VGLUT1 Antibody (2754) [NBP2-46627] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue
Immunohistochemistry: VGLUT1 Antibody (2754) [NBP2-46627] - Staining of rat brain shows strong positivity in glutamatergic processes in the molecular and granular layers of cerebellum.
Immunohistochemistry-Paraffin: VGLUT1 Antibody (2754) [NBP2-46627] - Staining of human cerebral cortex shows strong positivity in neuropil.
Immunohistochemistry-Paraffin: VGLUT1 Antibody (2754) [NBP2-46627] - Staining of human tonsil shows no positivity in lymphoid cells as expected.
Immunohistochemistry: VGLUT1 Antibody (2754) [NBP2-46627] - Staining of mouse hippocampus shows strong positivity in glutamatergic fibers in the CA2 layer.
Immunohistochemistry: VGLUT1 Antibody (2754) [NBP2-46627] - Staining of mouse brain shows strong positivity in glutamatergic fibers in the molecular and granular layers of the cerebellum.
Immunohistochemistry: VGLUT1 Antibody (2754) [NBP2-46627] - Staining of rat brain shows strong positivity in glutamatergic fibers in the polymorph layer of dentate gyrus.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
CL2754
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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VGLUT1 Antibody (CL2754) Summary

Immunogen
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence PSISEEERKYIEDAIGESAKLMNPLTKFSTP
Epitope
EERKYIEDAI
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SLC17A7
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
VGLUT1 Recombinant Protein Antigen (NBP2-46627PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for VGLUT1 Antibody (CL2754)

  • BNPI
  • BNPIbrain-specific Na-dependent inorganic phosphate cotransporter
  • Brain-specific Na(+)-dependent inorganic phosphate cotransporter
  • member 7
  • SLC17A7
  • solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter)
  • Solute carrier family 17 member 7
  • VGLUT1
  • VGLUT1vesicular glutamate transporter 1

Background

VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SLC17A7
Entrez
Uniprot