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VEGFR3/Flt-4 Antibody [Phycoerythrin]

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bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse VEGF R3/Flt-4 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB743P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.
bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse VEGF R3/Flt-4 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB743P, filled histogram) or isotype control antibody (Catalog # ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clonality
Polyclonal
Host
Goat
Conjugate
Phycoerythrin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 488
FAB743G
Allophycocyanin
FAB743A
Biotin
BAF743
Unconjugated
AF743
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF743
Carrier Free
AF743
Carrier Free
FAB743A
Carrier Free
FAB743G
Carrier Free
AF743-SP
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VEGFR3/Flt-4 Antibody [Phycoerythrin] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse VEGF R3/Flt-4
Tyr25-Asp770
Accession # P35917
Specificity
Detects mouse VEGF R3/Flt-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) VEGF R3, 10% cross-reactivity with rhVEGF R2, and 5% cross-reactivity with rhVEGF R1.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FLT4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR3/Flt-4 Antibody [Phycoerythrin]

  • EC 2.7.10
  • EC 2.7.10.1
  • FLT4
  • Flt-4
  • fms-related tyrosine kinase 4
  • LMPH1A
  • PCLFLT41
  • soluble VEGFR3 variant 1
  • soluble VEGFR3 variant 2
  • soluble VEGFR3 variant 3
  • Tyrosine-protein kinase receptor FLT4
  • vascular endothelial growth factor receptor 3
  • VEGF R3
  • VEGFR3
  • VEGFR-3
  • VEGFR3Fms-like tyrosine kinase 4

Background

VEGF R3 (Flt-4), together with VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1), belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of these receptors is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.

In adults, VEGF R3 expression is restricted to the endothelial cells of the lymphatic vessels. Mouse VEGF R3 cDNA encodes a 1363 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGF R3 has a 751 aa residue extracellular domain, a 22 aa residue hydrophobic transmembrane domain and a 565 aa residue cytoplasmic domain. The polypeptide sequences of murine Flt-4 is 88% identical to the human homologue. VEGF R3 has been reported to serve as the receptors for VEGF-C and VEGF-D.

  1. Finnerty, H. et al. (1993) Oncogene 8:2293.
  2. Joukov, V. et al. (1996) EMBO J. 15:290.
  3. Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol FLT4
Uniprot