Reactivity | MuSpecies Glossary |
Applications | WB, Flow, ELISA(Det) |
Clonality | Polyclonal |
Host | Goat |
Conjugate | Biotin |
Concentration | LYOPH |
Conjugate | Catalog # | Availability | Size | Price |
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Alexa Fluor 488 | FAB743G | |||
Allophycocyanin | FAB743A | |||
Phycoerythrin | FAB743P | |||
Unconjugated | AF743 | |||
Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse VEGFR3/Flt-4 Tyr25-Asp770 Accession # P35917 |
Specificity | Detects mouse VEGFR3 in ELISAs and Western blots. In sandwich ELISAs, less than 7% cross-reactivity with recombinant human VEGFR3 is observed and 0.2% cross-reactivity with recombinant mouse (rm) VEGFR1 and rmVEGFR2 is observed. |
Source | N/A |
Isotype | IgG |
Clonality | Polyclonal |
Host | Goat |
Gene | FLT4 |
Purity Statement | Antigen Affinity-purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | ELISA Capture: Mouse VEGF R3/Flt-4 Antibody (Catalog # MAB7431) ELISA Detection: Mouse VEGF R3/Flt-4 Biotinylated Antibody (Catalog # BAF743) Standard: Recombinant Mouse VEGF R3/Flt-4 Fc Chimera (Catalog # 743-R3) |
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Readout System | ||
Publications |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Preservative | No Preservative |
Concentration | LYOPH |
Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
VEGFR3 (Flt-4), together with VEGFR1 (Flt-1) and VEGFR2 (KDR/Flk-1), belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of these receptors is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.
In adults, VEGFR3 expression is restricted to the endothelial cells of the lymphatic vessels. Mouse VEGFR3 cDNA encodes a 1363 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGFR3 has a 751 aa residue extracellular domain, a 22 aa residue hydrophobic transmembrane domain, and a 565 aa residue cytoplasmic domain. The polypeptide sequences of murine Flt-4 is 88% identical to the human homologue. VEGFR3 has been reported to serve as the receptors for VEGF-C and VEGF-D.
Secondary Antibodies |
Isotype Controls |
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