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TWEAK R/TNFRSF12 Antibody

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Immunocytochemistry/Immunofluorescence: TWEAK R/TNFRSF12 Antibody [NBP1-85770] - Staining of human cell line U-251 MG shows localization to plasma membrane & cytosol. Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: TWEAK R/TNFRSF12 Antibody [NBP1-85770] - Staining of human cell line U-251 MG shows localization to plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TWEAK R/TNFRSF12 Antibody [NBP1-85770] - Staining of human prostate shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: TWEAK R/TNFRSF12 Antibody [NBP1-85770] - Staining of human kidney shows moderate membranous positivity in cells in tubules.
Immunohistochemistry-Paraffin: TWEAK R/TNFRSF12 Antibody [NBP1-85770] - Staining of human stomach shows strong membranous positivity in glanular cells.
Immunohistochemistry-Paraffin: TWEAK R/TNFRSF12 Antibody [NBP1-85770] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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TWEAK R/TNFRSF12 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RRCRRREKFTTPIEETGGEGCPAVALIQ
Predicted Species
Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TNFRSF12A
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
TWEAK R/TNFRSF12 Protein (NBP1-85770PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TWEAK R/TNFRSF12 Antibody

  • CD266 antigen
  • CD266
  • FGF-inducible 14
  • Fibroblast growth factor-inducible immediate-early response protein 14
  • Fn14
  • FN14tumor necrosis factor receptor superfamily member 12A
  • TNFRSF12
  • TNFRSF12A
  • tumor necrosis factor receptor superfamily, member 12A
  • TWEAK R
  • TWEAKR
  • tweak-receptor
  • type I transmembrane protein Fn14

Background

TWEAK (otherwise known as TNFRSF12A, Tumor necrosis factor receptor superfamily member 12A), is a member of the TNF family and was first described as a weak inducer of apoptosis in some cell types. It is thought to promote angiogenesis and the proliferation of endothelial cells as well as modulating cellular adhesion to matrix proteins. Recently, a receptor for TWEAK was isolated by expression cloning from a HUVEC cell cDNA library.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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TWEAK R/TNFRSF12
Antibody
NBP1-85770
TWEAK R/TNFRSF12 Antibody
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Applications: ICC/IF, IHC

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TNFRSF12A
Uniprot