![Western Blot: TRIM2 Antibody [NB100-1218] - Using 0.5ug/ml of this antibody, the staining of Rat Brain (A) + Peptide (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/TRIM2-Antibody-NB100-1218-img0005.jpg "Western Blot: TRIM2 Antibody [NB100-1218] - Using 0.5ug/ml of this antibody, the staining of Rat Brain (A) + Peptide (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
| Reactivity | Hu, Mu, Rt, Bv, CaSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence DSGNHCFKVYRYLQ, from the C Terminus of the protein sequence according to NP_056086.2; NP_001123539.1; NP_001337983.1; NP_001337984.1; NP_001337985.1l; NP_001337986.1. |
| Epitope | C-DSGNHCFKVYRYLQ |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_056086.2; NP_001123539.1). Cross-reactivity with TRIM3 has been demonstrated. |
| Predicted Species | Bovine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | TRIM2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | IP successful usage on mouse protein is reported in scientific literature (PMID: 21478148). WB: ~ 75-85kDa bands observed in rat brain lysates (calculated MW of 81.5 kDa band according to human NP_001123539.1, 84.4 according to human NP_056086.2 and 83.3 according to rat NP_001102022.1). An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human brain (cortex) shows cytoplasm stainning in neuronal cell bodies. Use in ICC/IF reported in secitific publication PMID: 32698497 |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | TRIM2 |
| Entrez | |
| Uniprot |
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![Immunohistochemistry-Paraffin: TRIM2 Antibody [NB100-1218] - Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/TRIM2-Antibody-Immunohistochemistry-Paraffin-NB100-1218-img0003.jpg "Immunohistochemistry-Paraffin: TRIM2 Antibody [NB100-1218] - Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Flow Cytometry: TRIM2 Antibody [NB100-1218] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/TRIM2-Antibody-Flow-Cytometry-NB100-1218-img0006.jpg "Flow Cytometry: TRIM2 Antibody [NB100-1218] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
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