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Thrombomodulin/BDCA-3 Antibody - BSA Free

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Western Blot: Thrombomodulin/BDCA-3 Antibody [NBP1-88133] - Analysis in human lung tissue.
Immunocytochemistry/ Immunofluorescence: Thrombomodulin/BDCA-3 Antibody [NBP1-88133] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody [NBP1-88133] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody [NBP1-88133] - Analysis in human lung and cerebral cortex tissues. Corresponding THBD RNA-seq data are presented for the same ...read more
Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody [NBP1-88133] - Staining of human cerebral cortex shows no positivity in neurons as expected.
Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody [NBP1-88133] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody [NBP1-88133] - Staining of human lung shows strong membranous positivity in pneumocytes.
Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody [NBP1-88133] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody [NBP1-88133] - Staining of human lung shows strong membranous positivity in pneumocytes and endothelial cells.
Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody [NBP1-88133] - Staining of human pancreas shows strong membranous positivity in endothelial cells.
Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody [NBP1-88133] - Staining of human placenta shows moderate membranous positivity in trophoblastic and endothelial cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Validated by:
       

Orthogonal Strategies

 

Order Details

Thrombomodulin/BDCA-3 Antibody - BSA Free Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DVISLLLNGDGGVGRRRLWIGLQLPPGCGDPKRLGPLRGFQWVTGDNNTSYSRWARLDLNGAPLCGPLCVAVSAAEATVPSEPIWEEQQCEVKADGFLCEFHFPATCRPLAVEPGAAAAAVSITYG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
THBD
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Thrombomodulin/BDCA-3 Protein (NBP1-88133PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Thrombomodulin/BDCA-3 Antibody - BSA Free

  • BDCA3
  • BDCA-3
  • CD141 antigen
  • CD141
  • Fetomodulin
  • THBD
  • THRM
  • Thrombomodulin
  • TM

Background

CD141/Thrombomodulin is an exclusively endothelial cell surface glycoprotein that forms a 1:1 complex with thrombin. Binding of thrombin to this high affinity receptor alters its specificity toward several substrates. The complex activates protein C approximately 1000 times faster than thrombin alone. Activated protein C degrades clotting factors V and VIII; thus, thrombomodulin converts thrombin into a physiologic anticoagulant. Thrombomodulin is also found in the circulatory and urinary systems, the physiologic significance of this is obscure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Research Areas for Thrombomodulin/BDCA-3 Antibody (NBP1-88133)

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Blogs on Thrombomodulin/BDCA-3.

Thrombomodulin - A multifunctional protein with roles in inflammation and coagulation
Thrombomodulin, also known as BDCA-3, is a glycosylated transmembrane protein present on the surface of vascular endothelial cells. Thrombomodulin is a high-affinity receptor for thrombin, a key protein in the coagulation cascade. Formation of the...  Read full blog post.

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Bioinformatics

Gene Symbol THBD