![Western Blot: Syndecan-1/CD138 Antibody (JM11-21) [NBP2-67174] - Analysis of Syndecan 1 on NIH/3T3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Syndecan-1-CD138-Antibody-JM11-21-Western-Blot-NBP2-67174-img0009.jpg "Western Blot: Syndecan-1/CD138 Antibody (JM11-21) [NBP2-67174] - Analysis of Syndecan 1 on NIH/3T3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | JM11-21 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Recombinant protein within Human Syndecan-1/CD138 aa 13-274 / 310. (SwissProt: P18827 Human; SwissProt: P18828 Mouse; SwissProt: P26260 Rat) |
| Localization | Membrane, Secreted. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | SDC1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Simple Western CD44 Antibody (2C5) [Unconjugated] - s Pan Specific](https://images.novusbio.com/images2/CD44_BBA10_Simple_Western_21377.jpg)
![Western Blot CD44 Antibody (2C5) [Unconjugated] - s Pan Specific](https://images.novusbio.com/images2/CD44_BBA10_Western_Blot_21459.jpg)
![Immunohistochemistry CD44 Antibody (2C5) [Unconjugated] - s Pan Specific](https://images.novusbio.com/images2/CD44_BBA10_Immunohistochemistry_17361.jpg)
| Publication using NBP2-67174 | Applications | Species |
|---|---|---|
| Abdullah S, Ghio M, Cotton-Betteridge A et al. Succinate metabolism and membrane reorganization drives the endotheliopathy and coagulopathy of traumatic hemorrhage Science advances 2023-06-16 [PMID: 37315138] (IP, Human) | IP | Human |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SDC1 |
![Immunocytochemistry/Immunofluorescence: Syndecan-1/CD138 Antibody (JM11-21) [NBP2-67174] - Staining Syndecan 1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Syndecan-1-CD138-Antibody-JM11-21-Immunocytochemistry-Immunofluorescence-NBP2-67174-img0003.jpg "Immunocytochemistry/Immunofluorescence: Syndecan-1/CD138 Antibody (JM11-21) [NBP2-67174] - Staining Syndecan 1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Syndecan-1/CD138 Antibody (JM11-21) [NBP2-67174] - Analysis of paraffin-embedded mouse prostate tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Syndecan-1-CD138-Antibody-JM11-21-Immunohistochemistry-Paraffin-NBP2-67174-img0008.jpg "Immunohistochemistry-Paraffin: Syndecan-1/CD138 Antibody (JM11-21) [NBP2-67174] - Analysis of paraffin-embedded mouse prostate tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.")
![Immunocytochemistry/Immunofluorescence: Syndecan-1/CD138 Antibody (JM11-21) [NBP2-67174] - Staining Syndecan 1 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Syndecan-1-CD138-Antibody-JM11-21-Immunocytochemistry-Immunofluorescence-NBP2-67174-img0001.jpg "Immunocytochemistry/Immunofluorescence: Syndecan-1/CD138 Antibody (JM11-21) [NBP2-67174] - Staining Syndecan 1 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: Syndecan-1/CD138 Antibody (JM11-21) [NBP2-67174] - Staining Syndecan 1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Syndecan-1-CD138-Antibody-JM11-21-Immunocytochemistry-Immunofluorescence-NBP2-67174-img0002.jpg "Immunocytochemistry/Immunofluorescence: Syndecan-1/CD138 Antibody (JM11-21) [NBP2-67174] - Staining Syndecan 1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Syndecan-1/CD138 Antibody (JM11-21) [NBP2-67174] - Analysis of paraffin-embedded human kidney tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Syndecan-1-CD138-Antibody-JM11-21-Immunohistochemistry-Paraffin-NBP2-67174-img0004.jpg "Immunohistochemistry-Paraffin: Syndecan-1/CD138 Antibody (JM11-21) [NBP2-67174] - Analysis of paraffin-embedded human kidney tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Syndecan-1/CD138 Antibody (JM11-21) [NBP2-67174] - Analysis of paraffin-embedded human lung tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Syndecan-1-CD138-Antibody-JM11-21-Immunohistochemistry-Paraffin-NBP2-67174-img0005.jpg "Immunohistochemistry-Paraffin: Syndecan-1/CD138 Antibody (JM11-21) [NBP2-67174] - Analysis of paraffin-embedded human lung tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Syndecan-1/CD138 Antibody (JM11-21) [NBP2-67174] - Analysis of paraffin-embedded human tonsil tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Syndecan-1-CD138-Antibody-JM11-21-Immunohistochemistry-Paraffin-NBP2-67174-img0006.jpg "Immunohistochemistry-Paraffin: Syndecan-1/CD138 Antibody (JM11-21) [NBP2-67174] - Analysis of paraffin-embedded human tonsil tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Syndecan-1/CD138 Antibody (JM11-21) [NBP2-67174] - Analysis of paraffin-embedded mouse colon tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Syndecan-1-CD138-Antibody-JM11-21-Immunohistochemistry-Paraffin-NBP2-67174-img0007.jpg "Immunohistochemistry-Paraffin: Syndecan-1/CD138 Antibody (JM11-21) [NBP2-67174] - Analysis of paraffin-embedded mouse colon tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.")
![Western Blot Syndecan-2/CD362 Antibody [Unconjugated]](https://images.novusbio.com/images2/Syndecan-2_AF6585_Western_Blot_11618.jpg)
![Immunohistochemistry Syndecan-2/CD362 Antibody [Unconjugated]](https://images.novusbio.com/images2/Syndecan-2_AF6585_Immunohistochemistry_11640.jpg)
![Flow Cytometry Syndecan-2/CD362 Antibody [Unconjugated]](https://images.novusbio.com/images2/Syndecan-2_AF6585_Flow_Cytometry_11674.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![Immunohistochemistry CD45 Antibody [Unconjugated]](https://images.novusbio.com/images2/CD45_AF114_Immunohistochemistry_23525.jpg)
![Immunocytochemistry CD45 Antibody [Unconjugated]](https://images.novusbio.com/images2/af114_mouse-cd45-affinity-purified-polyclonal-ab-immunocytochemistry-6122021145449.jpg)
![Immunocytochemistry/ Immunofluorescence CD45 Antibody [Unconjugated]](https://images.novusbio.com/images/af114_mouse-cd45-affinity-purified-polyclonal-ab-41202410331954.jpg)
![Immunohistochemistry Neprilysin/CD10 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neprilysin_AF1126_Immunohistochemistry_6679.jpg)
![Western Blot Neprilysin/CD10 Antibody [Unconjugated]](https://images.novusbio.com/images/af1126_mouse-neprilysin-affinity-purified-polyclonal-ab-8220249505976.jpg)
![Western Blot Neprilysin/CD10 Antibody [Unconjugated]](https://images.novusbio.com/images/af1126_mouse-neprilysin-affinity-purified-polyclonal-ab-44202415433122.jpg)
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![Immunohistochemistry Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody [Unconjugated]](https://images.novusbio.com/images2/Endorepellin_AF2364_Immunohistochemistry_6744.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")