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Synaptophysin Antibody (SP15)

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Western Blot: Synaptophysin Antibody (SP15) [NBP1-97459] - Analysis of rat liver tissue extract, probed with Synaptophysin, mAb (SP15).

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, IHC
Clone
SP15
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Synaptophysin Antibody (SP15) Summary

Immunogen
Synaptic vesicle-containing fractions immunoprecipitated from human brain homogenates using anti-human Synaptophysin mAb antibodies.
Marker
Pre-synaptic marker
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
SYP
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:10000
Application Notes
This antibody is useful in Immunohistochemistry and Western blot (Detects a band of ~38kDa).
Publications
Read Publications using
NBP1-97459 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Purity
Ammonium sulfate precipitation

Alternate Names for Synaptophysin Antibody (SP15)

  • Major synaptic vesicle protein p38
  • MRX
  • MRXSYP
  • Synaptophysin
  • SYP

Background

Synaptophysin is an integral membrane protein involved in neurotransmitter exocytosis. This protein consists of four transmembrane domains, with its N- and C-terminus facing the cytoplasm. Studies have shown Synaptophysin to be a major cholesterol-binding protein in brain synaptic vesicles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Synaptophysin Antibody (NBP1-97459)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IF/IHC, WB.


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IF/IHC
(1)
WB
(1)
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Mouse
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Synaptophysin Antibody (NBP1-97459) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Synaptophysin Products

Research Areas for Synaptophysin Antibody (NBP1-97459)

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Blogs on Synaptophysin.

Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease
 By Jamshed Arslan Pharm.D.  Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amy...  Read full blog post.

Synaptophysin a Marker Protein in Neuroendocrine Cells
Synaptophysin a Marker Protein in Neuroendocrine Cells Synaptophysin is a major integral membrane glycoprotein of neuronal synaptic vesicles present in virtually all synapses and shows a high degree of evolutionary conservation across the mammals. Syn...  Read full blog post.

Characterizing Synaptophysin is "a Snap"
Synaptophysin is an integral membrane glycoprotein found within the small synaptic vesicles in brain and endocrine cells. Studies with synaptophysin antibodies show that it is one of the most abundant small vesicle proteins, constituting approximately...  Read full blog post.

Synaptophysin and Dementing Disorders
 Synaptophysin (a presynaptic vesicle protein) is an integral membrane glycoprotein originally isolated from presynaptic vesicles of bovine neurons. Synaptophysin is found in all nerve terminals and synaptophysin measurements have been used to quantif...  Read full blog post.

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Bioinformatics

Gene Symbol SYP