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Synaptophysin Antibody (7H12) [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, IHC
Clone
7H12
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-47483AF350
Alexa Fluor 405
NBP1-47483AF405
Alexa Fluor 488
NBP1-47483AF488
Alexa Fluor 532
NBP1-47483AF532
Alexa Fluor 594
NBP1-47483AF594
Alexa Fluor 647
NBP1-47483AF647
Alexa Fluor 700
NBP1-47483AF700
Alexa Fluor 750
NBP1-47483AF750
Biotin
NBP1-47483B
DyLight 350
NBP1-47483UV
DyLight 405
NBP1-47483V
DyLight 488
NBP1-47483G
DyLight 550
NBP1-47483R
DyLight 594
NBP1-47483DL594
DyLight 650
NBP1-47483C
DyLight 680
NBP1-47483FR
DyLight 755
NBP1-47483IR
FITC
NBP1-47483F
HRP
NBP1-47483H
Janelia Fluor 549
NBP1-47483JF549
Janelia Fluor 646
NBP1-47483JF646
Unconjugated
NBP1-47483
mFluor Violet 450 SE
NBP1-47483MFV450
mFluor Violet 500 SE
NBP1-47483MFV500
mFluor Violet 610 SE
NBP1-47483MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-47483
BSA Free
NBP1-47483
BSA Free
NBP1-47483SS
Azide and BSA Free
NBP2-80972
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Synaptophysin Antibody (7H12) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Purified recombinant fragment of human Synaptophysin expressed in E. coli. [UniProt# P08247]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SYP
Purity
Ammonium sulfate precipitation
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Ammonium sulfate precipitation

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Synaptophysin Antibody (7H12) [CoraFluor™ 1]

  • Major synaptic vesicle protein p38
  • MRX
  • MRXSYP
  • Synaptophysin
  • SYP

Background

Synaptophysin (Syp I), also called the major synaptic vesicle protein p38, is an integral membrane glycoprotein found in the synaptic vesicles of brain, neuron and neuroendocrine cells. Synaptophysin is one of the most abundant membrane proteins, comprising approximately 7% of the total protein in small synaptic vesicles. In mature nerve terminals it forms a complex with the vesicular membrane protein synaptobrevin, which appears to modulate synaptobrevin's interaction with the plasma membrane-associated proteins syntaxin and SNAP25 to form the SNARE complex as a prerequisite for membrane fusion. Synaptophysin may also be used as a neuroendocrine tumor marker in both neuroscience and cancer research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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 By Jamshed Arslan Pharm.D.  Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amy...  Read full blog post.
Synaptophysin a Marker Protein in Neuroendocrine Cells
Synaptophysin a Marker Protein in Neuroendocrine Cells Synaptophysin is a major integral membrane glycoprotein of neuronal synaptic vesicles present in virtually all synapses and shows a high degree of evolutionary conservation across the mammals. Syn...  Read full blog post.
Characterizing Synaptophysin is "a Snap"
Synaptophysin is an integral membrane glycoprotein found within the small synaptic vesicles in brain and endocrine cells. Studies with synaptophysin antibodies show that it is one of the most abundant small vesicle proteins, constituting approximately...  Read full blog post.
Synaptophysin and Dementing Disorders
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Bioinformatics

Gene Symbol SYP