Home » ST6 GalNAc alpha-2,6-sialyltransferaseV/ST6GALNAC5 » ST6GALNAC5 Antibodies » ST6 GalNAc alpha-2,6-sialyltransferaseV/ST6GALNAC5 Antibody (719526)
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ST6 GalNAc alpha-2,6-sialyltransferaseV/ST6GALNAC5 Antibody (719526)

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Western blot shows lysates of MDA-MB-231 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GalNAc a-2,6-sialyltransferase V/ST6GALNAC5 Monoclonal Antibody (Catalog # MAB6715) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GalNAc a-2,6-sialyltransferase V/ST6GALNAC5 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of MDA-MB-231 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GalNAc a-2,6-sialyltransferase V/ST6GALNAC5 Monoclonal Antibody (Catalog # MAB6715) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
719526
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB6715-SP
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ST6 GalNAc alpha-2,6-sialyltransferaseV/ST6GALNAC5 Antibody (719526) Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human GalNAc alpha -2,6-sialyltransferase V/ST6GALNAC5
Gly30-Phe336
Accession # Q9BVH7
Specificity
Detects human GalNAc alpha -2,6-sialyltransferase V/ST6GALNAC5 in direct ELISAs. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ST6GALNAC4 or rhST6GALNAC6 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ST6GALNAC5
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ST6 GalNAc alpha-2,6-sialyltransferaseV/ST6GALNAC5 Antibody (719526)

  • alpha-N-acetylneuraminyl 2,3-betagalactosyl-1,3)-N-acetyl galactosaminidealpha-2,6-sialyltransferase E
  • EC 2.4.99
  • EC 2.4.99.-
  • EC 2.4.99.7
  • GalNAc alpha-2,6-sialyltransferase V
  • GD1 alpha synthase
  • MGC3184
  • sialyltransferase 7 (alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase) E
  • sialyltransferase 7((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminidealpha-2,6-sialyltransferase) E
  • Sialyltransferase 7E
  • SIAT7E
  • SIAT7-E
  • SIAT7Ealpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase V
  • ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminidealpha-2,6-sialyltransferase 5
  • ST6 neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminidealpha-2,6-sialyltransferase 5
  • ST6GalNAc V
  • ST6GALNAC5
  • ST6GalNAcV
  • ST6GalNAcValpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 5

Background

Gangliosides are acidic glycosphingolipids that contain one or more sialic acid residues (1). They are abundant in the nervous system, where they play crucial modulatory roles in cellular recognition, interaction, adhesion, and signal transduction, particularly during early developmental stages. The expression of gangliosides in the nervous system is developmentally regulated through various sialyltransferases (2). ST6GALNAC5 is a sialyltransferase involved in the biosynthesis of ganglioside GD1a (NeuAc alpha 2,3Gal beta 1,3GalNAc beta 1,4(NeuAc alpha 2,3)Gal beta 1,4Glc beta 1-Cer) from GM1b (NeuAc alpha 2,3Gal beta 1,3GalNAc beta 1,4Gal beta 1,4Glc beta 1-Cer), and its expression is restricted to the brain (3, 4). ST6GALNAC5 has been identified as a key player in the metastasis of breast cancer cells to the brain by potentially enabling the cancer cells to cross the blood-brain barrier (5, 6). The recombinant ST6GALNAC5 was active on fetuin from fetal calf serum when assayed using a phosphatase-coupled method (7) suggesting the substrate specificity of ST6GALNAC5 may require further characterization.
  1. Kolter, T. et al. (2002) J. Biol. Chem. 277:25859.
  2. Yu, R.K. et al. (2008) Glycoscience DOI: 10.1007/978-3-540-30429-6_41.
  3. Okajima, T. et al. (1999) J. Biol. Chem.274:30557.
  4. Harduin-Lepers, A. et al. (2005) Glycobiology 15:805.
  5. Bos, P.D. et al. (2009) Nature 459:1005.
  6. Arshad, F. et al. (2011). Patholog. Res. Int. DOI: 10.4061/2011/920509.
  7. Wu, Z.L. et al. (2010) Glycobiology DOI: 10.1093/glycob/cwq187.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ST6GALNAC5
Uniprot