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Mouse Serpin C1/Antithrombin-III ELISA Kit (Colorimetric)

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ELISA: Mouse Serpin C1/Antithrombin-III ELISA Kit (Colorimetric) [NBP2-78765] - Samples were spiked with high concentrations of Mouse Serpin C1/Antithrombin-III and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Mouse Serpin C1/Antithrombin-III ELISA Kit (Colorimetric) [NBP2-78765] - Samples were spiked with high concentrations of Mouse Serpin C1/Antithrombin-III and diluted with Reference Standard & Sample Diluent to ...read more
ELISA: Mouse Serpin C1/Antithrombin-III ELISA Kit (Colorimetric) [NBP2-78765] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids.
Standard Curve Range
3.13- 200 ng/mL
Sensitivity
1.88 ng/mL

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Mouse Serpin C1/Antithrombin-III ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse AT-III in samples. No Significant cross-reactivity or interference between Mouse AT-III and analogues was observed.
Standard Curve Range
3.13- 200 ng/mL
Sensitivity
1.88 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.03%
Intra-Assay
CV% < 4.80
Spike Recovery
87-95%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
SERPINC1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Mouse Serpin C1/Antithrombin-III ELISA Kit (Colorimetric)

  • Antithrombin-III
  • AT3antithrombin-III
  • ATIII
  • ATIIIantithrombin III
  • MGC22579
  • serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1
  • serine-cysteine proteinase inhibitor clade C member 1
  • Serpin C1
  • serpin peptidase inhibitor, clade C (antithrombin), member 1

Background

Antithrombin III (ATH III) is a plasma protein synthesised in the liver. Normal plasma levels are 115 to 160mg/L. ATH III consists of a single polypeptide chain and has a molecular weight of 58kDa. ATH III is a serine protease inhibitor and regulates coagulation by the formation of covalently liked complexes. ATH III also has a heparin binding site and in the presence of heparin the inhibition of thrombin, factors IXa, Xa and XIa is enhanced 1000 fold. Low levels of ATH III (functional or concentration) are associated with deep vein thrombosis and pulmonary embolism. Antithrombin assays are based on immunologic, enzymatic, or clotting properties

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol SERPINC1