Orthogonal Strategies: Western Blot: SEC24C Antibody [NBP1-81550] - Analysis in human cell lines PC-3 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Independent Antibodies: Western Blot: SEC24C Antibody [NBP1-81550] - Analysis using Anti-SEC24C antibody NBP1-81550 (A) shows similar pattern to independent antibody NBP1-81549 (B).
Immunocytochemistry/ Immunofluorescence: SEC24C Antibody [NBP1-81550] - Staining of human cell line U-251 MG shows localization to nucleoplasm and vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SEC24C Antibody [NBP1-81550] - Staining of human stomach, lower shows moderate granular cytoplasmic positivity in glandular cells.
This antibody was developed against Recombinant Protein corresponding to amino acids: RLPSMTGPLLPGQSFGGPSVSQPNHVSSPPQALPPGTQMTGPLGPLPPMHSPQQPGYQPQQNGSFGPARGPQSNYGGPYPAAPTF
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SEC24C
Purity
Immunogen affinity purified
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Reactivity reported in scientific literature (PMID: 24743550)
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for SEC24C Antibody
KIAA0079SEC24-related protein C
protein transport protein Sec24C
SEC24 (S. cerevisiae) related gene family, member C
SEC24 family, member C (S. cerevisiae)
SEC24 related gene family, member C
Background
The protein encoded by the SEC24C gene is a member of the SEC24 subfamily of the SEC23/SEC24 family, which is involved in vesicle trafficking. The encoded protein has similarity to yeast Sec24p component of COPII. COPII is the coat protein complex responsible for vesicle budding from the ER. The product of this gene may play a role in shaping the vesicle, as well as in cargo selection and concentration. Alternatively spliced transcript variants encoding the same protein have been identified. (provided by RefSeq)
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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