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S100A6 Antibody [Unconjugated]

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Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/ Mouse S100A6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4584) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for S100A6 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/ Mouse S100A6 Antigen ...read more
S100A6 was detected in immersion fixed paraffin-embedded sections of human kidney using Sheep Anti-Human/Mouse S100A6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4584) at 3 µg/mL overnight at 4 °C. ...read more
Simple Western lane view shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line and RAW 264.7 mouse monocyte/macrophage cell line, loaded at 0.2 mg/mL. A specific band was detected for S100A6 at approximately 7 ...read more
Genetic Strategies: Western blot shows lysates of HEK293T human embryonic kidney parental cell line and S100A6 knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IHC, KO
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF4584
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4584-SP
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S100A6 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse S100A6
Met1-Lys89
Accession # P14069
Specificity
Detects human and mouse S100A6 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse S100A1 and recombinant mouse S100A4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
S100A6
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Knockout Validated
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF4584 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S100A6 Antibody [Unconjugated]

  • 2A9
  • CABP
  • CACY
  • CACY5B10
  • calcyclin
  • Growth factor-inducible protein 2A9
  • MLN 4
  • PRA
  • PRAS100 calcium binding protein A6 (calcyclin)
  • Prolactin receptor-associated protein
  • protein S100-A6
  • S100 calcium binding protein A6
  • S100 calcium-binding protein A6 (calcyclin)
  • S100 calcium-binding protein A6
  • S100A6

Background

Mouse S100A6 (also prolactin receptor-associated protein and calcyclin) is a 10 kDa member of the S100 family of calcium-binding proteins. S100A6 is 89 amino acids (aa) in length and contains two calcium-binding EF-hand domains (aa 12‑47 and 48‑83). Intracellularly, S100A6 will both noncovalently homodimerize and heterodimerize with S100B plus SGT1. Extracellularly, it is secreted via a noncanonical pathway and binds to RAGE, inducing apoptosis. It is expressed by neurons, endothelium, fibroblasts and glandular epithelia. Mouse S100A6 is 99% and 96% aa identical to rat and human S100A6, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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S100A6 Antibody
[Unconjugated]
AF4584
S100A6 Antibody [Unconjugated]
Species: Hu, Mu
Applications: WB, Simple Western, IHC, KO

Publications for S100A6 Antibody (AF4584)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Immunohistochemistry, Plasma Culture, Western Blot.


Filter By Application
Immunohistochemistry
(1)
Plasma Culture
(1)
Western Blot
(1)
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(1)
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(2)
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Secondary Antibodies

 

Isotype Controls

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Blogs on S100A6.

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S100A6: Playing Roles in Cancer, Apoptosis & Transcription Regulation
S100A6 antibodies detect a small calcium binding protein with 2 EF-hand structures and belongs to the S100 family. Calcium binding induces a conformational change of the protein which in turn permits its interaction with several target proteins. It is...  Read full blog post.
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Bioinformatics

Gene Symbol S100A6
Uniprot