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Recombinant Mouse Lymphotoxin alpha1/beta2 Protein

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Recombinant Mouse Lymphotoxin alpha 1/ beta 2 (Catalog # 9968-LY)induces NIH‑3T3 mouse embryonic fibroblast cell proliferation. TheED50 for this effect is 0.3-2.1 ng/mL.
Recombinant Mouse Lymphotoxin alpha 1/ beta 2 (Catalog # 9968-LY)induces NIH‑3T3 mouse embryonic fibroblast cell proliferation. TheED50 for this effect is 0.3-2.1 ng/mL.
2 μg/lane of Recombinant Mouse Lymphotoxin alpha1/beta2 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 42-63 kDa.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

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Catalog# & Formulation Size Price
Standard Formulation
9968-LY-025/CF
Carrier Free
9968-LY-025/CF
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Recombinant Mouse Lymphotoxin alpha1/beta2 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.3-2.1 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Lymphotoxin protein
Mouse LT alpha
(Lys59-Leu202)
Accession # P09225		GGGGS
Mouse LT beta
(Leu153-Gly306) Accession # P41155
GGGGS
Mouse LT beta
(Leu153-Gly306) Accession # P41155
Accession #
N-terminal Sequence
Lys59
Structure / Form
GS-linked heterotrimer
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
42-63 kDa, reducing conditions
Publications
Read Publication using
9968-LY in the following applications:

Packaging, Storage & Formulations

Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Lymphotoxin alpha1/beta2 Protein

  • LT
  • Lymphotoxin alpha
  • Lymphotoxin
  • tnfb
  • TNFSF1
  • TNLG1E

Background

Lymphotoxin alpha (LT alpha) and Lymphotoxin beta (LT beta) are pro-inflammatory TNF superfamily ligands that play important roles in immune system development (1, 2). Mouse LT alpha cDNA encodes a 202 amino acid (aa) secreted soluble protein with a 33 aa signal sequence. The mature mouse LT alpha shares 75% aa sequence identity with human LT alpha (3, 4). Mouse LT beta cDNA encodes a 306 aa type II membrane protein with a 27 aa N-terminal cytoplasmic domain, a 21 aa transmembrane region, and a 258 aa extracellular domain. It shares 73% aa sequence identity with human LT beta within common regions of their extracellular domains (5). Secreted LT alpha assembles as a soluble homotrimer, LT alpha 3. In addition, secreted LT alpha also complexes with the membrane associated LT beta to generate two types of heterotrimers, LT alpha 1/beta 2 and LT alpha 2/beta 1 (5). The soluble LT alpha 3 binds both TNF RI (p55) and TNF RII (p75). In contrast, the predominant membrane-bound heterotrimer, LT alpha 1/beta 2, binds only to the lymphotoxin beta receptor (LT beta R). LT alpha 2/beta 1 is capable of binding LT beta R, TNF RI (p55), and TNF RII (p75). LT plays a role in normal lymphoid organogenesis (6, 7). The LT alpha 1/beta 2 heterotrimer binds and activates the LT beta R/TNFRSF3 which is expressed on macrophages, dendritic cells, hepatocytes, intestinal epithelial cells (IEC), follicular dendritic cells (FDC), and high endothelial venules (HEV) (2, 8, 9). LT beta R also serves as a receptor for LIGHT/TNFSF14 (10). LT alpha 1/beta 2 promotes the development of FDC networks and HEV in lymphoid tissue, the class switching of immature B cells for IgA production, and the production of homeostatic IL-22 by ILCs (11-14). It can be shed by ADAM17 or MMP-8 mediated cleavage, and the released heterotrimer circulates in the serum and is elevated in synovial fluid of rheumatoid arthritis patients (15).
  1. Lu, T.T. and J.L. Browning (2014) Front. Immunol. 5:47.
  2. Upadhyay, V. and Y.-X. Fu (2014) Cytokine Growth Factor Rev. 25:227.
  3. Gray, P.W. et al. (1984) Nature 312:721.
  4. Nedwin, G.E. et al. (1985) J. Cell Biochem. 29:171.
  5. Browning, J.L. et al. (1993) Cell 72:847.
  6. Ettinger R. et al. (1996) Proc. Natl. Acad. Sci. USA 93:13102.
  7. Cuff, C.A. et al. (1998) J. Immunol. 161:6853.
  8. Sudhamsu, J. et al. (2013) Proc. Natl. Acad. Sci. USA 110:19896.
  9. Crowe, P.D. et al. (1994) Science 264:707.
  10. Eldredge, J. et al. (2006) Biochemistry 45:10117.
  11. Kruglov, A.A. et al. (2013) Science 342:1243.
  12. Futterer, A. et al. (1998) Immunity 9:59.
  13. Koni, P.A. et al. (1997) Immunity 6:491.
  14. Ota, N. et al. (2011) Nat. Immunol. 12:941.
  15. Young, J. et al. (2010) Cytokine 51:78.

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Publications for Lymphotoxin (9968-LY)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Bioassay.


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