Name: Recombinant Mouse CXCL2/MIP-2 Protein
Brand: Novus Biologicals
SKU: 452-M2
Rating: 5 (1 reviews)

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Cell migration/motility CXCL2/GRO beta/MIP-2/CINC-3 452-M2

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reviewed by:
James Xiao

Cell migration/motility

04/17/2018

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Summary

Application	Cell migration/motility
Lot	PF0314092

Comments

A total of 10^6 mouse neutrophils was added to an insert of a transwell. Recombinant mouse MIP-2 (100 ng/ml) was used as a neutrophil chemoattractant and was added to the lower chamber. After 2 hours of incubation, migrated neutrophils in the lower chambers were enumerated.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	MuSpecies Glossary
Applications	Bioactivity

Catalog# & Formulation	Size	Price
Carrier Free 452-M2-010/CF

Carrier Free 452-M2-050/CF

Details of Functionality	Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED₅₀ for this effect is 0.500‑5.00 ng/mL.
Source	E. coli-derived mouse CXCL2/GRO beta/MIP-2/CINC-3 protein Ala28-Asn100
Accession #	P10889
N-terminal Sequence	Ala28
Protein/Peptide Type	Recombinant Proteins
Gene	Cxcl2
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note	<0.01 EU per 1 μg of the protein by the LAL method.

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Recombinant Mouse CXCL2/MIP-2 Protein

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Recombinant Mouse CXCL2/MIP-2 Protein Summary

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Alternate Names for Recombinant Mouse CXCL2/MIP-2 Protein

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Publications for CXCL2/GRO beta/MIP-2/CINC-3 (452-M2)(49)

Review for CXCL2/GRO beta/MIP-2/CINC-3 (452-M2) (1) 51

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Recent Reviews

Bioinformatics

Publications using 452-M2	Applications	Species
Pajulas, A;Fu, Y;Cheung, CCL;Chu, M;Cannon, A;Alakhras, N;Zhang, J;Ulrich, BJ;Nelson, AS;Zhou, B;Kaplan, MH; Interleukin-9 promotes mast cell progenitor proliferation and CCR2-dependent mast cell migration in allergic airway inflammation Mucosal immunology 2023-05-10 [PMID: 37172907] (Bioassay, Mouse)	Bioassay	Mouse
N Cheng, KH Kim, LF Lau Senescent hepatic stellate cells promote liver regeneration through IL-6 and ligands of CXCR2 JCI Insight, 2022-06-16;0(0):. 2022-06-16 [PMID: 35708907] (In Vivo, Mouse)	In Vivo	Mouse
ML Capitano, Y Sammour, J Ropa, M Legendre, N Mor-Vaknin, DM Markovitz DEK, a nuclear protein, is chemotactic for hematopoietic stem/progenitor cells acting through CXCR2 and Galphai signaling Journal of leukocyte biology, 2022-02-08;0(0):. 2022-02-08 [PMID: 35137444] (Bioassay, Mouse)	Bioassay	Mouse
JM Adrover, A Aroca-Crev, G Crainiciuc, F Ostos, Y Rojas-Vega, A Rubio-Ponc, C Cilloniz, E Bonzón-Kul, E Calvo, D Rico, MA Moro, C Weber, I Lizasoaín, A Torres, J Ruiz-Cabel, J Vázquez, A Hidalgo Programmed 'disarming' of the neutrophil proteome reduces the magnitude of inflammation Nat. Immunol., 2020-01-13;21(2):135-144. 2020-01-13 [PMID: 31932813] (Bioassay, Mouse)	Bioassay	Mouse
S Sharif, N Ueda, Y Nakatani, LM Wise, S Clifton, Z Lateef, AA Mercer, SB Fleming Chemokine-Binding Proteins Encoded by Parapoxvirus of Red Deer of New Zealand Display Evidence of Gene Duplication and Divergence of Ligand Specificity Front Microbiol, 2019-06-25;10(0):1421. 2019-06-25 [PMID: 31293551] (Binding Assay, Mouse)	Binding Assay	Mouse
L Hao, X Lei, H Zhou, AJ Marshall, L Liu Critical role for PI3K?-dependent neutrophil reactive oxygen species in WKYMVm-induced microvascular hyperpermeability J. Leukoc. Biol., 2019-06-19;0(0):. 2019-06-19 [PMID: 31216371] (In Vivo, Mouse, Transgenic Mouse)	In Vivo	Mouse, Transgenic Mouse
ML Capitano, N Mor-Vaknin, AK Saha, S Cooper, M Legendre, H Guo, R Contreras-, F Kappes, MA Sartor, CT Lee, X Huang, DM Markovitz, HE Broxmeyer Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling J. Clin. Invest., 2019-05-20;130(0):2555-2570. 2019-05-20 [PMID: 31107242] (Bioassay, Mouse)	Bioassay	Mouse
W Li, G Feng, JM Gauthier, I Lokshina, R Higashikub, S Evans, X Liu, A Hassan, S Tanaka, M Cicka, HM Hsiao, D Ruiz-Perez, A Bredemeyer, RW Gross, DL Mann, YY Tyurina, AE Gelman, VE Kagan, A Linkermann, KJ Lavine, D Kreisel Ferroptotic cell death and TLR4/Trif signaling initiate neutrophil recruitment after heart transplantation J. Clin. Invest., 2019-02-26;130(0):. 2019-02-26 [PMID: 30830879] (Bioassay, Mouse)	Bioassay	Mouse
VY Su, KY Yang, SH Chiou, NJ Chen, MH Mo, CS Lin, CT Wang Induced Pluripotent Stem Cells Regulate TREM-1 Expression and the p38 MAPK Pathway in Endotoxin-Induced Acute Lung Injury Stem Cells, 2019-02-16;0(0):. 2019-02-16 [PMID: 30681755] (Bioassay, Mouse)	Bioassay	Mouse
M Hossain, E Omran, N Xu, L Liu The Specific Mitogen- and Stress-Activated Protein Kinase MSK1 Inhibitor SB-747651A Modulates Chemokine-Induced Neutrophil Recruitment Int J Mol Sci, 2017-10-17;18(10):. 2017-10-17 [PMID: 29039777] (In Vivo, Mouse)	In Vivo	Mouse
Clara Jana-Lui Busch Malondialdehyde epitopes are sterile mediators of hepatic inflammation in hypercholesterolemic mice Hepatology, 2017-02-03;0(0):. 2017-02-03 [PMID: 27981604] (ELISA (Standard))	ELISA (Standard)
Pedro Luiz Rosalen Main pathways of action of Brazilian red propolis on the modulation of neutrophils migration in the inflammatory process Phytomedicine, 2016-09-28;23(13):1583-1590. 2016-09-28 [PMID: 27823622] (Bioassay, Mouse)	Bioassay	Mouse
Hossain M, Qadri S, Xu N, Su Y, Cayabyab F, Heit B, Liu L Endothelial LSP1 Modulates Extravascular Neutrophil Chemotaxis by Regulating Nonhematopoietic Vascular PECAM-1 Expression. J Immunol, 2015-08-03;195(5):2408-16. 2015-08-03 [PMID: 26238489] (In Vivo, Mouse)	In Vivo	Mouse
Spahn J, Li W, Bribriesco A, Liu J, Shen H, Ibricevic A, Pan J, Zinselmeyer B, Brody S, Goldstein D, Krupnick A, Gelman A, Miller M, Kreisel D DAP12 expression in lung macrophages mediates ischemia/reperfusion injury by promoting neutrophil extravasation. J Immunol, 2015-03-11;194(8):4039-48. 2015-03-11 [PMID: 25762783] (Bioassay, In Vivo, Mouse)	Bioassay, In Vivo	Mouse
Germena G, Volmering S, Sohlbach C, Zarbock A Mutation in the CD45 inhibitory wedge modulates integrin activation and leukocyte recruitment during inflammation. J Immunol, 2014-12-10;194(2):728-38. 2014-12-10 [PMID: 25505282] (Bioassay, Mouse)	Bioassay	Mouse
Li S, Pettersson U, Hoorelbeke B, Kolaczkowska E, Schelfhout K, Martens E, Kubes P, Van Damme J, Phillipson M, Opdenakker G Interference with glycosaminoglycan-chemokine interactions with a probe to alter leukocyte recruitment and inflammation in vivo. PLoS ONE, 2014-08-05;9(8):e104107. 2014-08-05 [PMID: 25093679] (Bioassay, Mouse)	Bioassay	Mouse
Sonego F, Castanheira F, Czaikoski P, Kanashiro A, Souto F, Franca R, Nascimento D, Freitas A, Spiller F, Cunha L, Zamboni D, Alves-Filho J, Cunha F MyD88-, but not Nod1- and/or Nod2-deficient mice, show increased susceptibility to polymicrobial sepsis due to impaired local inflammatory response. PLoS ONE, 2014-08-01;9(8):e103734. 2014-08-01 [PMID: 25084278] (In Vivo, Mouse)	In Vivo	Mouse
Tancevski I, Nairz M, Duwensee K, Auer K, Schroll A, Heim C, Feistritzer C, Hoefer J, Gerner R, Moschen A, Heller I, Pallweber P, Li X, Theurl M, Demetz E, Wolf A, Wolf D, Eller P, Ritsch A, Weiss G Fibrates ameliorate the course of bacterial sepsis by promoting neutrophil recruitment via CXCR2. EMBO Mol Med, 2014-04-06;6(6):810-20. 2014-04-06 [PMID: 24755316] (Bioassay, Mouse)	Bioassay	Mouse
Fujihara Y, Takato T, Hoshi K Macrophage-inducing FasL on chondrocytes forms immune privilege in cartilage tissue engineering, enhancing in vivo regeneration. Stem Cells, 2014-05-01;32(5):1208-19. 2014-05-01 [PMID: 24446149] (Bioassay, Human, Mouse)	Bioassay	Human, Mouse
Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis. J Immunol, 2013-04-15;190(10):5329-36. 2013-04-15 [PMID: 23589623] (ELISA (Standard), Mouse)	ELISA (Standard)	Mouse
Zhang Q, Coveney A, Yu S, Liu J, Li Y, Blankson S, Redmond H, Wang J, Wang J Inefficient antimicrobial functions of innate phagocytes render infant mice more susceptible to bacterial infection. Eur J Immunol, 2013-03-22;43(5):1322-32. 2013-03-22 [PMID: 23404483] (Bioassay, Mouse)	Bioassay	Mouse
Spiller F, Carlos D, Souto FO, de Freitas A, Soares FS, Vieira SM, Paula FJ, Alves-Filho JC, Cunha FQ alpha-Acid glycoprotein decreases neutrophil migration and increases susceptibility to sepsis in diabetic mice. Diabetes, 2012-03-13;61(6):1584-91. 2012-03-13 [PMID: 22415874] (In Vivo, Mouse)	In Vivo	Mouse
Sadik CD, Kim ND, Alekseeva E, Luster AD IL-17RA signaling amplifies antibody-induced arthritis. PLoS ONE, 2011-10-20;6(10):e26342. 2011-10-20 [PMID: 22028860] (Bioassay, Mouse)	Bioassay	Mouse
Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines. Am. J. Pathol., 2011-10-01;179(6):2730-9. 2011-10-01 [PMID: 21967814] (ELISA (Standard))	ELISA (Standard)
Filardy AA, Pires DR, Nunes MP, Takiya CM, Freire-de-Lima CG, Ribeiro-Gomes FL, DosReis GA Proinflammatory clearance of apoptotic neutrophils induces an IL-12(low)IL-10(high) regulatory phenotype in macrophages. J. Immunol., 2010-07-21;185(4):2044-50. 2010-07-21 [PMID: 20660352] (In Vivo, Mouse)	In Vivo	Mouse
Massena S, Christoffersson G, Hjertstrom E, Zcharia E, Vlodavsky I, Ausmees N, Rolny C, Li JP, Phillipson M A chemotactic gradient sequestered on endothelial heparan sulfate induces directional intraluminal crawling of neutrophils. Blood, 2010-06-08;116(11):1924-31. 2010-06-08 [PMID: 20530797] (Bioassay, Mouse)	Bioassay	Mouse
Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production. Proc. Natl. Acad. Sci. U.S.A., 2009-03-16;106(14):5954-9. 2009-03-16 [PMID: 19289819] (Bioassay, Mouse)	Bioassay	Mouse
Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis. Microbes Infect., 2009-03-13;11(4):524-30. 2009-03-13 [PMID: 19286469] (ELISA (Standard))	ELISA (Standard)
Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis. Immunology, 2008-04-03;125(2):272-9. 2008-04-03 [PMID: 18393969] (ELISA (Standard))	ELISA (Standard)
Shojaei F, Wu X, Zhong C, Yu L, Liang XH, Yao J, Blanchard D, Bais C, Peale FV, van Bruggen N, Ho C, Ross J, Tan M, Carano RA, Meng YG, Ferrara N Bv8 regulates myeloid-cell-dependent tumour angiogenesis. Nature, 2007-12-06;450(7171):825-31. 2007-12-06 [PMID: 18064003] (Bioassay, Mouse)	Bioassay	Mouse
Santen S, Schramm R, Menger MD, Wang Y, Jeppsson B, Thorlacius H P-selectin glycoprotein ligand-1 regulates chemokine-dependent leukocyte recruitment in colonic ischemia-reperfusion. Inflamm. Res., 2007-11-01;56(11):452-8. 2007-11-01 [PMID: 17891336] (In Vivo, Mouse)	In Vivo	Mouse
Kile BT, Panopoulos AD, Stirzaker RA, Hacking DF, Tahtamouni LH, Willson TA, Mielke LA, Henley KJ, Zhang JG, Wicks IP, Stevenson WS, Nurden P, Watowich SS, Justice MJ Mutations in the cofilin partner Aip1/Wdr1 cause autoinflammatory disease and macrothrombocytopenia. Blood, 2007-05-21;110(7):2371-80. 2007-05-21 [PMID: 17515402] (Bioassay, Mouse)	Bioassay	Mouse
Liu L, Puri KD, Penninger JM, Kubes P Leukocyte PI3Kgamma and PI3Kdelta have temporally distinct roles for leukocyte recruitment in vivo. Blood, 2007-05-08;110(4):1191-8. 2007-05-08 [PMID: 17488877] (Bioassay, Mouse)	Bioassay	Mouse
Zemann B, Urtz N, Reuschel R, Mechtcheriakova D, Bornancin F, Badegruber R, Baumruker T, Billich A Normal neutrophil functions in sphingosine kinase type 1 and 2 knockout mice. Immunol. Lett., 2007-01-30;109(1):56-63. 2007-01-30 [PMID: 17292973] (Bioassay, In Vivo, Mouse)	Bioassay, In Vivo	Mouse
Fan X, Patera AC, Pong-Kennedy A, Deno G, Gonsiorek W, Manfra DJ, Vassileva G, Zeng M, Jackson C, Sullivan L, Sharif-Rodriguez W, Opdenakker G, Van Damme J, Hedrick JA, Lundell D, Lira SA, Hipkin RW Murine CXCR1 is a functional receptor for GCP-2/CXCL6 and interleukin-8/CXCL8. J. Biol. Chem., 2006-12-29;282(16):11658-66. 2006-12-29 [PMID: 17197447] (Binding Assay, Mouse)	Binding Assay	Mouse
Watanabe H, Kubo M, Numata K, Takagi K, Mizuta H, Okada S, Ito T, Matsukawa A Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis. J. Immunol., 2006-12-15;177(12):8650-7. 2006-12-15 [PMID: 17142765] (ELISA (Standard))	ELISA (Standard)
Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors. Infect. Immun., 2006-09-18;74(12):6730-8. 2006-09-18 [PMID: 16982824] (ELISA (Standard))	ELISA (Standard)
Panopoulos AD, Zhang L, Snow JW, Jones DM, Smith AM, El Kasmi KC, Liu F, Goldsmith MA, Link DC, Murray PJ, Watowich SS STAT3 governs distinct pathways in emergency granulopoiesis and mature neutrophils. Blood, 2006-08-03;108(12):3682-90. 2006-08-03 [PMID: 16888100] (Bioassay, Mouse)	Bioassay	Mouse
Ramos CD, Fernandes KS, Canetti C, Teixeira MM, Silva JS, Cunha FQ Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4). Eur. J. Immunol., 2006-08-01;36(8):2025-34. 2006-08-01 [PMID: 16856209] (ELISA (Standard))	ELISA (Standard)
Osuchowski MF, Welch K, Siddiqui J, Remick DG Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality. J. Immunol., 2006-08-01;177(3):1967-74. 2006-08-01 [PMID: 16849510] (ELISA (Standard))	ELISA (Standard)
Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury. J. Immunol., 2006-07-01;177(1):612-20. 2006-07-01 [PMID: 16785559] (ELISA (Standard))	ELISA (Standard)
Rhodes KE, Raivich G, Fawcett JW The injury response of oligodendrocyte precursor cells is induced by platelets, macrophages and inflammation-associated cytokines. Neuroscience, 2006-04-21;140(1):87-100. 2006-04-21 [PMID: 16631314] (In Vivo, Rat)	In Vivo	Rat
Wislez M, Fujimoto N, Izzo JG, Hanna AE, Cody DD, Langley RR, Tang H, Burdick MD, Sato M, Minna JD, Mao L, Wistuba I, Strieter RM, Kurie JM High expression of ligands for chemokine receptor CXCR2 in alveolar epithelial neoplasia induced by oncogenic kras. Cancer Res., 2006-04-15;66(8):4198-207. 2006-04-15 [PMID: 16618742] (Bioassay, Mouse)	Bioassay	Mouse
Mihara K, Smit MJ, Krajnc-Franken M, Gossen J, Rooseboom M, Dokter W Human CXCR2 (hCXCR2) takes over functionalities of its murine homolog in hCXCR2 knockin mice. Eur. J. Immunol., 2005-09-01;35(9):2573-82. 2005-09-01 [PMID: 16094689] (In Vivo, Mouse)	In Vivo	Mouse
Osuchowski MF, Siddiqui J, Copeland S, Remick DG Sequential ELISA to profile multiple cytokines from small volumes. J. Immunol. Methods, 2005-07-01;302(1):172-81. 2005-07-01 [PMID: 16023134] (ELISA (Standard))	ELISA (Standard)
Jeyaseelan S, Chu HW, Young SK, Worthen GS Transcriptional profiling of lipopolysaccharide-induced acute lung injury. Infect. Immun., 2004-12-01;72(12):7247-56. 2004-12-01 [PMID: 15557650] (ELISA Development, Mouse)	ELISA Development	Mouse
Cotena A, Platt N The class A macrophage scavenger receptor attenuates CXC chemokine production and the early infiltration of neutrophils in sterile peritonitis. J. Immunol., 2004-11-15;173(10):6427-32. 2004-11-15 [PMID: 15528383] (In Vivo, Mouse)	In Vivo	Mouse
Ueno T, Kuse S, Saito F, Nitta T, Kakiuchi T, Hollander GA, Takahama Y The role of CCL21 in recruitment of T-precursor cells to fetal thymi. Blood, 2004-09-09;105(1):31-9. 2004-09-09 [PMID: 15358618] (Bioassay, Mouse)	Bioassay	Mouse
Fulton SA, Reba SM, Martin TD, Boom WH Neutrophil-mediated mycobacteriocidal immunity in the lung during Mycobacterium bovis BCG infection in C57BL/6 mice. Infect. Immun., 2002-09-01;70(9):5322-7. 2002-09-01 [PMID: 12183593] (In Vivo, Mouse)	In Vivo	Mouse
Show All 49 Publications. Collapse Publications.

	James Xiao 04/17/2018
Application:	Cell migration/motility
Species:

Gene Symbol	Cxcl2
Uniprot	P10889()