Recombinant Human Nectin-1 His-tag Avi-tag Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Nectin-1, also called poliovirus receptor-related protein-1 (PRR1), is a member of the Nectin family which are Ca++-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions. The Nectin family is comprised of 4 family members and 5 nectin-like molecules and they are structurally homologous to the poliovirus receptors (1). Mature human Nectin-1 consists of an extracellular domain (ECD) with three immunoglobulin-like domains, a single transmembrane segment, and a cytoplasmic domain that interacts with the F-actin–binding protein afadin (2). Within the ECD, human Nectin-1 shares 93% and 94% amino acid sequence identity with mouse and rat Nectin1, respectively. Two splice variants of Nectin-1 are known, one with an alternate cytoplasmic domain and a soluble form (4). Nectin-1 binds viral glycoprotein D to mediate herpesvirus (but not poxvirus) entry into vaginal mucosa, sensory neurons and fibroblasts (4-7). In forming adherens junctions and synapses, Nectins-1 and -3 initiate cell-cell interactions, recruiting alpha v beta 3 integrin extracellularly and cadherins intracellularly through afadin and other junctional proteins (2, 8-11). Nectin-1 forms homodimers in cis, followed by interactions in trans with Nectin-1, -3 or -4 (1). These interactions organize the cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections. Nectin-1 also recognizes CD96 on NK cells (12). Deficiency of Nectin-1 can result in cleft lip/palate ectodermal dysplasia (13). Nectin-1 down-regulation in epithelial cancers, mediated in part by ectodomain shedding, may contribute to invasiveness (14). In colorectal cancer, Nectin-1 expression was found to be associated with a high risk for early disease recurrence (15). Our Avi-tag Biotinylated Nectin-1 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

Array AVI2880

• Nectin-1/PVRL1 Antibodies
• Nectin-1/PVRL1 Antibody Pairs
• Nectin-1/PVRL1 ELISA Kits
• Nectin-1/PVRL1 Lysate
• Nectin-1/PVRL1 Protein
• Nectin-1/PVRL1 Proteins and Enzymes