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Recombinant Human M-CSF R/CD115 Fc Avi-tag Protein, CF

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When Human M-CSF (Catalog # 216-MC) is immobilzed at 1 μg/mL (100 μL/well), Biotinylated Recombinant Human M‑CSF R/CD115 Fc Chimera Avi-tag (Catalog # AVI10339) binds with an ED50 of 6-36 ng/mL.
When Human M-CSF (Catalog # 216-MC) is immobilzed at 1 μg/mL (100 μL/well), Biotinylated Recombinant Human M‑CSF R/CD115 Fc Chimera Avi-tag (Catalog # AVI10339) binds with an ED50 of 6-36 ng/mL.
2 μg/lane of Biotinylated Recombinant Human M-CSF R/CD115 Fc Chimera Avi-tag (Catalog # AVI10339) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue ...read more

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
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Carrier-Free

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Recombinant Human M-CSF R/CD115 Fc Avi-tag Protein, CF Summary

Additional Information
Biotinylated
Details of Functionality
The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Human M‑CSF (Catalog # 216-MC) is immobilized at 1 µg/mL (100 µL/well), it binds to Biotinylated Recombinant Human M‑CSF R/CD115 Fc Chimera Avi-tag (Catalog # AVI10339) with an ED50 of 6-36 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human M-CSF R/CD115 protein
Human M-CSF R/CD115
(Ile20-Thr516)
Accession # P07333.2		IEGRMDHuman IgG1
(Pro100-Lys330)		Avi-tag
N-terminusC-terminus
Accession #
N-terminal Sequence
Ile20
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Bioactivity2
Theoretical MW
83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
100-120 kDa, under reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human M-CSF R/CD115 Fc Avi-tag Protein, CF

  • CD115 antigen
  • CD115
  • c-fms
  • colony stimulating factor 1 receptor
  • CSF1R
  • CSF-1-R
  • CSFR
  • EC 2.7.10.1
  • FMS proto-oncogene
  • FMSFIM2
  • macrophage colony stimulating factor I receptor
  • macrophage colony-stimulating factor 1 receptor
  • McDonough feline sarcoma viral (v-fms) oncogene homolog
  • M-CSF R
  • MCSFR
  • M-CSFR
  • Proto-oncogene c-Fms

Background

M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region (1-4). M-CSF receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells and in the developing placenta (1). Human M-CSF receptor cDNA encodes a 972 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 493 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain and a 435 aa cytoplasmic domain. The human M-CSF R ECD shares 60%, 64%, 72%, 75%, 75% and 76% aa identity with mouse, rat, bovine, canine, feline and equine M-CSF R, respectively. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF receptor, releasing the functional ligand-binding extracellular domain (5). M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction (6). The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK and c-Cbl are key transducers of M-CSF R signals (3, 4). M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts and DC (3, 4). M-CSF R and integrin  alpha v beta 3 share signaling pathways during osteoclastogenesis and deletion of either causes osteopetrosis (7, 8). In the brain, microglia expressing increased
M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear (9, 10).

  1. deParseval, N. et al. (1993) Nucleic Acids Res. 21:750.
  2. Rothwell, V.M. and L.R. Rohrschneider (1987) Oncogene Res. 1:311.
  3. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
  4. Ross, F.P. and S.L. Teitelbaum (2005) Immunol. Rev. 208:88.
  5. Rovida, E. et al. (2001) J. Immunol. 166:1583.
  6. Yeung, Y. et al. (1998) J. Biol. Chem. 273:17128.
  7. Dai, X. et al. (2002) Blood 99:111.
  8. Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
  9. Li, M. et al. (2004) J. Neurochem. 91:623.
  10. Mitrasinovic, O.M. et al. (2005) J. Neurosci. 25:4442.

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