Recombinant Human CD44v5 Fc Chimera Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

CD44 is a ubiquitously expressed protein that is the major receptor for hyaluronan and exerts control over cell growth and migration (1‑3). Human CD44 has a 20 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan‑binding disulfide‑stabilized link region and a 325‑530 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. CD44 transcripts undergo complex alternative splicing, and, within the stem, ten variably spliced exons (v1‑10 corresponding to exons 6‑15; although human CD44 lacks v1/exon 6) produce multiple protein isoforms (1‑4). The standard or hematopoietic form, CD44H, does not include the variable segments (1‑4). Cancer aggressiveness and T cell activation have been correlated with expression of specific isoforms (1, 4, 5). Human CD44v5 contains exon 10 and is associated with tumor progression and metastasis in many types of cancer including breast, colon, lung, renal, skin, and ovarian tumors (6, 12). With variable N‑ and O‑glycosylation and splicing within the stalk, CD44 can range from 80 to 200 kDa (1). Within the N‑terminal invariant portion of the ECD (aa 21‑222), human CD44 shares 76%, 76%, 86%, 83% and 79% identity with corresponding mouse, rat, equine, canine and bovine CD44, respectively. The many reported functions of CD44 fall within three categories (1). First, CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a "platform" for growth factors and metalloproteinases. Second, CD44 can function as a co‑receptor that modifies activity of receptors including MET and the ERBB family of tyrosine kinases. Third, the CD44 intracellular domain links the plasma membrane to the actin cytoskeleton via the ERM proteins, ezrin, radixin and moesin. CD44 can be synthesized in a soluble form (6) or may be cleaved at multiple sites by either membrane‑type matrix metalloproteinases, or ADAM proteases to produce soluble ectodomains (8, 9). The cellular portion may then undergo gamma secretase‑dependent intramembrane cleavage to form an A beta‑like transmembrane portion and a cytoplasmic signaling portion that affects gene expression (10, 11). These cleavage events are thought to promote metastasis by enhancing tumor cell motility and growth (1, 8). CD44v5 plays important role in cancer progression involving in cell migration and invasion and is considered a functional cancer biomarker (6, 12).

Array 10146-CD

• CD44 Antibodies
• CD44 Antibody Pair
• CD44 ELISA Kits
• CD44 Lysates
• CD44 Protein
• CD44 ELISA
• CD44 Proteins and Enzymes