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Recombinant Human CD40/TNFRSF5 Fc Chimera Protein, CF

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When Recombinant Human CD40/TNFRSF5 Fc Chimera (Catalog # 1493-CDB) isimmobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Human CD40 Ligand/TNFSF5 (Catalog # 6420-CL) that produces 50% optimal binding is 4-24 ng/mL.
When Recombinant Human CD40/TNFRSF5 Fc Chimera (Catalog # 1493-CDB) isimmobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Human CD40 Ligand/TNFSF5 (Catalog # 6420-CL) that produces 50% optimal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human CD40/TNFRSF5 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human CD40/TNFRSF5 Fc Chimera is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human CD40 Ligand/TNFSF5 (Catalog # 6420-CL) that produces 50% optimal binding is 4-24 ng/mL. Measured by its ability to inhibit rhCD40 Ligand-induced IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human CD40. The ED50 for this effect is 0.08-0.8 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human CD40/TNFRSF5 protein
Human CD40
(Glu21-Arg193)
Accession # P25942-1		DIEGRMDHuman IgG1
(Pro100-Lys330) 		6-His tag
N-terminusC-terminus
Accession #
N-terminal Sequence
Glu21
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Bioactivity2
Theoretical MW
47 kDa .
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
58-66 kDa, reducing conditions
Publications
Read Publications using
1493-CDB in the following applications:

Packaging, Storage & Formulations

Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CD40/TNFRSF5 Fc Chimera Protein, CF

  • B cell surface antigen CD40
  • B-cell surface antigen CD40
  • Bp50B cell-associated molecule
  • CD40 antigen
  • CD40 molecule, TNF receptor superfamily member 5
  • CD40 type II isoform
  • CD40
  • CD40L receptor
  • CDw40
  • MGC9013
  • nerve growth factor receptor-related B-lymphocyte activation molecule
  • p50
  • TNFRSF5
  • TNFRSF5CD40 antigen (TNF receptor superfamily member 5)
  • tumor necrosis factor receptor superfamily member 5
  • tumor necrosis factor receptor superfamily, member 5

Background

CD40, also known as TNFRSF5, is a 45-50 kDa type I transmembrane glycoprotein member of the TNF receptor superfamily (1). Mature human CD40 consists of a 173 amino acid (aa) extracellular domain, a transmembrane domain, and a 62 aa cytoplasmic domain (2). The extracellular domain of human CD40 shares 58% and 56% aa sequence identity with mouse and rat CD40, respectively. An antagonistic soluble human CD40 splice variant contains an alternate sequence within the extracellular and transmembrane domains and lacks a cytoplasmic domain (3). CD40 is expressed on the surface of B cells, dendritic cells, macrophages, monocytes and platelets, as well as endothelial and epithelial cells (4, 5). Interaction of CD40 with its ligand, CD40 Ligand, leads to the aggregation of CD40 molecules resulting in the initiation of bidirectional intracellular signaling in both CD40 and CD40 Ligand expressing cells (6). CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses (7, 8). CD40 serves multiple functions in both hematopoietic and epithelial cancers and is a target for tumor immunotherapy (9, 10). Dysregulation of CD40/CD40 Ligand expression and interactions contributes to the immune deficiency associated with HIV infection and AIDS (11, 12). It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis (13, 14).
  1. Banchereau, J. et al. (1994) Annu. Rev. Immunol. 12:881.
  2. Stamenkovic, I. et al. (1989) EMBO J. 8:1403.
  3. Eshel, D. et al. (2008) Mol. Immunol. 46:250.
  4. van Kooten, C. and J. Banchereau (1997) Curr. Opin. Immunol. 9:330.
  5. Schonbeck, U. et al. (1997) J. Biol. Chem. 272:19569.
  6. Eissner, G. et al. (2004) Cytokine Growth Factor Rev. 15:353.
  7. Rickert, R.C. et al. (2011) Immunol. Rev. 244:115.
  8. Elgueta, R. et al. (2009) Immunol. Rev. 229:152.
  9. Loskog, A.S. and A.G. Eliopoulos (2009) Semin. Immunol. 21:301.
  10. Hangalapura, B.N. et al. (2012) J. Gene Med. 14:416.
  11. Kornbluth, R.S. (2000) J. Leukoc. Biol. 68:373.
  12. Chougnet, C. (2003) J. Leukoc. Biol. 74:702.
  13. Pamukcu, B. et al. (2011) Ann. Med. 43:331.
  14. Hassan, G.S. et al. (2012) Immunobiology 217:521.

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Recombinant Human
CD40/TNFRSF5 Fc Chime ...
1493-CDB
Recombinant Human CD40/TNFRSF5 Fc Chimera Protein, CF
Species: Hu
Applications: Bioactivity

Publications for CD40/TNFRSF5 (1493-CDB)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Bioassay, Surface Plasmon Resonance (SPR.


Filter By Application
Bioassay
(4)
Surface Plasmon Resonance (SPR
(1)
All Applications
Filter By Species
Human
(5)
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Showing Publications 1 - 5 of 5.
Publications using 1493-CDB Applications Species
X Yu, HTC Chan, CM Orr, O Dadas, SG Booth, LN Dahal, CA Penfold, L O'Brien, CI Mockridge, RR French, P Duriez, LR Douglas, AR Pearson, MS Cragg, I Tews, MJ Glennie, AL White Complex Interplay between Epitope Specificity and Isotype Dictates the Biological Activity of Anti-human CD40 Antibodies Cancer Cell, 2018-03-22;0(0):. 2018-03-22 [PMID: 29576376] (Surface Plasmon Resonance (SPR, Human) Surface Plasmon Resonance (SPR Human
FH Al-Awadhi, VJ Paul, H Luesch Structural Diversity and Anticancer Activity of Marine-Derived Elastase Inhibitors: Key Features and Mechanisms Mediating the Antimetastatic Effects in Invasive Breast Cancer Chembiochem, 2018-03-23;0(0):. 2018-03-23 [PMID: 29405541] (Bioassay, Human) Bioassay Human
Magri G, Miyajima M, Bascones S, Mortha A, Puga I, Cassis L, Barra C, Comerma L, Chudnovskiy A, Gentile M, Llige D, Cols M, Serrano S, Arostegui J, Juan M, Yague J, Merad M, Fagarasan S, Cerutti A Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells. Nat Immunol, 2014-02-23;15(4):354-64. 2014-02-23 [PMID: 24562309] (Bioassay, Human) Bioassay Human
ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation. Blood, 2012-05-10;119(26):6268-77. 2012-05-10 [PMID: 22577174] (Bioassay, Human) Bioassay Human
Kaur K, Chowdhury S, Greenspan NS, Schreiber JR Decreased expression of tumor necrosis factor family receptors involved in humoral immune responses in preterm neonates. Blood, 2007-07-18;110(8):2948-54. 2007-07-18 [PMID: 17634409] (Bioassay, Human) Bioassay Human

Reviews for CD40/TNFRSF5 (1493-CDB) (0)

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FAQs for CD40/TNFRSF5 (1493-CDB). (Showing 1 - 2 of 2 FAQs).

  1. I wanna know does CD40 antibody for IHC-P comes with positive control slide?
    • Our CD40 antibodies do not come with positive control slides however you can use any type of primary carcinoma cell as a positive control. I recommend breast carcinoma as this is what we have tested our CD40 antibodies on.
  2. We are studying CD40L in our system. It has been known that both CD40 and CD11b are receptors for CD40L. We need CD40 antibody to neutralize CD40 binding to CD40L, then we may prove what is function for CD11b binding to CD40L. Do you have any CD40 Ab which has block function only between CD40 and CD40L, but not between CD11b and CD40L?
    • Unfortunately, none of our CD40 antibodies have been specifically tested for this application.

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