Home » CD31/PECAM-1 » CD31/PECAM-1 Proteins » Recombinant Human CD31/PECAM-1 Protein, CF
Novus Biologicals products are now on bio-techne.com

Recombinant Human CD31/PECAM-1 Protein, CF

 (2 Publications)
 See All Related
Submit a Review

Images

 
There are currently no images for CD31/PECAM-1 (ADP6).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Recombinant Human CD31/PECAM-1 Protein, CF Summary

Details of Functionality
Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 5 x 104 cells/well are added to rhCD31 coated plates (3.3 μg/mL, 100 μL/well) in the presence of 20 ng.mL PMA, approximately 50-65% will adhere after 30 minutes at 37°C.
Source
Chinese Hamster Ovary cell line, CHO-derived human CD31/PECAM-1 protein
Gln28-Lys601
Accession #
N-terminal Sequence
No results obtained: Gln28 predicted
Protein/Peptide Type
Recombinant Proteins
Gene
PECAM1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
64.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
ADP6 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS containing Calcium and Magnesium with Sorbitol.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 150 μg/mL in sterile, deionized water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CD31/PECAM-1 Protein, CF

  • adhesion molecule
  • CD31 antigen
  • CD31
  • CD31/EndoCAM
  • EndoCAM
  • FLJ34100
  • FLJ58394
  • GPIIA'
  • PECA1
  • PECAM1
  • PECAM-1
  • PECAM-1, CD31/EndoCAM
  • platelet endothelial cell adhesion molecule
  • platelet endothelial cell adhesion molecule-1
  • platelet/endothelial cell adhesion molecule

Background

The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alpha v beta 3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.

Customers Who Viewed This Item Also Viewed...

VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(683 Publications)
Species: Hu
Applications: ELISA
CD34 Antibody
[Unconjugated]
AF4117
Immunohistochemistry CD34 Antibody [Unconjugated]
(3 Reviews)
(37 Publications)
Species: Rt
Applications: IHC, WB
CTLA-4
[Unconjugated]
7268-CT
Bioactivity CTLA-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
HLA DQ/DR/DP
Antibody (HLA-Pan/296 ...
NBP2-79843
Protein Array HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Western Blot HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
NOD2 Antibody
(2D9) - BSA Free
NB100-524
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Immunohistochemistry-Frozen NOD2 Antibody (2D9) - BSA Free
(1 Review)
(26 Publications)
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
ICAM-1/CD54
Antibody [Unconjugate
AF796
Immunohistochemistry ICAM-1/CD54 Antibody [Unconjugated]
Western Blot ICAM-1/CD54 Antibody [Unconjugated]
Western Blot ICAM-1/CD54 Antibody [Unconjugated]
(6 Reviews)
(88 Publications)
Species: Mu
Applications: AdBlk, IHC, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(811 Publications)
Species: Hu
Applications: BA
Von Willebrand
Factor Antibody
NB600-586
Dual RNAscope ISH-IHC Von Willebrand Factor Antibody
Immunohistochemistry-Frozen Von Willebrand Factor Antibody
Immunohistochemistry Von Willebrand Factor Antibody
(3 Reviews)
(14 Publications)
Species: Ca, Fe, Hu, Mu
Applications: Dual ISH-IHC, ICC/IF, IHC, IHC-Fr, IHC-P
E-Selectin/CD62E
Antibody (BBIG-E4) [U ...
BBA16
Immunohistochemistry E-Selectin/CD62E Antibody (BBIG-E4) [Unconjugated]
Immunocytochemistry E-Selectin/CD62E Antibody (BBIG-E4) [Unconjugated]
Flow Cytometry E-Selectin/CD62E Antibody (BBIG-E4) [Unconjugated]
(1 Review)
(60 Publications)
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, WB
CD45 Antibody
[Unconjugated]
AF114
Immunohistochemistry CD45 Antibody [Unconjugated]
Immunocytochemistry CD45 Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence CD45 Antibody [Unconjugated]
(1 Review)
(70 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, ICC, IHC, WB
Endoglin/CD105
Antibody [Unconjugate
AF1320
Immunocytochemistry Endoglin/CD105 Antibody [Unconjugated]
Immunocytochemistry Endoglin/CD105 Antibody [Unconjugated]
Simple Western Endoglin/CD105 Antibody [Unconjugated]
(2 Reviews)
(44 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
VE-Cadherin
Antibody [Unconjugate
AF1002
Immunohistochemistry VE-Cadherin Antibody [Unconjugated]
Western Blot VE-Cadherin Antibody [Unconjugated]
Simple Western VE-Cadherin Antibody [Unconjugated]
(3 Reviews)
(118 Publications)
Species: Mu
Applications: Dual ISH-IHC, IHC, Simple Western, WB
FGF
basic/FGF2/bFGF [Unco
233-FB
FGF basic/FGF2/bFGF [Unconjugated]
(548 Publications)
Species: Hu
Applications: BA
VEGFR2/KDR/Flk-1
Antibody [Unconjugate ...
AF644
Neutralization VEGFR2/KDR/Flk-1 Antibody [Unconjugated]
Immunohistochemistry VEGFR2/KDR/Flk-1 Antibody [Unconjugated]
Immunohistochemistry VEGFR2/KDR/Flk-1 Antibody [Unconjugated]
(3 Reviews)
(98 Publications)
Species: Mu
Applications: CyTOF-ready, Dual ISH-IHC, Flow, IHC, Neut, WB
P-Selectin/CD62P
137-PS
P-Selectin/CD62P
(46 Publications)
Species: Hu
Applications: BA
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(661 Publications)
Species: Mu
Applications: ELISA
Vimentin Antibody
NB300-223
Immunocytochemistry/ Immunofluorescence Vimentin Antibody
Immunohistochemistry-Frozen Vimentin Antibody
Immunocytochemistry/ Immunofluorescence Vimentin Antibody
(6 Reviews)
(70 Publications)
Species: Bv, Ca, Ch, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
Factor VIII
Antibody - BSA Free
NB100-91761
Western Blot Factor VIII Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence Factor VIII Antibody - BSA Free
Western Blot Factor VIII Antibody - BSA Free
(5 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB

Publications for CD31/PECAM-1 (ADP6)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Bioassay, Western Blot.


Filter By Application
Bioassay
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for CD31/PECAM-1 (ADP6) (0)

There are no reviews for CD31/PECAM-1 (ADP6). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for CD31/PECAM-1 (ADP6) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CD31/PECAM-1 Products

Blogs on CD31/PECAM-1.

Check out the latest blog posts on CD31/PECAM-1.

  Read full blog post.
The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

CD31/PECAM-1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights&#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; NL001) and counterstained with DAPI (blue). Specific staining was localized to developing endothelium. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
CD31/PECAM-1 Antibody [Unconjugated]
AF3628
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human CD31/PECAM-1 Protein, CF and receive a gift card or discount.


Bioinformatics

Gene Symbol PECAM1
Uniprot