Recombinant Equine PDGF-BB Protein

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Platelet-Derived Growth Factor (PDGF)-BB is synthesized as a prepro-precurser that contains a signal peptide, an N-terminal prodomain, a mature region, and a
C-terminal prodomain (1-4). The proprecursor is initially dimerized and then intracellulary processed twice. The N-terminal prodomain is cleaved first, followed by cleavage of the C-terminal prodomain prior to the formation of a disulfide-linked homodimer. Mature equine PDGF-B shares 95%, 88%, 89%, 83%, 79%, and 79% amino acid sequence identity with human, mouse, rat, sheep, cow, and goat PDGF-B, respectively. PDGF-BB is expressed by hepatocytes and nonresorbing osteoclasts, generating osteoblasts and bone formation (4, 5). It is also produced by platelets, macrophages, and mast cells. At sites of injury, it promotes neutrophil and macrophage infiltration for debridement, fibroblast secretion of new extracellular matrix, and IGF-I-mediated re-epithelialization (6, 7). The traditional receptor for PDGF is either a homodimer or heterodimer created from two type I transmembrane RTKs, PDGF R alpha and PDGF R beta (8, 9). PDGF-BB has been shown to bind the alpha alpha  homodimer, alpha beta heterodimer, and the beta beta homodimer in vitro, and act through the beta beta homodimer in vivo (8, 10). Equine PDGF-BB has been shown to stimulate contraction of fibroblast-populated collagen matrix, a model of equine wound contraction, in a Rho kinase- and p38-dependent manner (12). PDGF-BB is present in platelet-rich plasma (PRP), which has been used to treat soft tissue injuries in horses (13, 14). The levels of equine PDGF-BB in PRP is dependent on breed, gender, and age (15).

Array 8585-BB

• PDGF-BB Antibodies
• PDGF-BB ELISA Kits
• PDGF-BB Proteins
• PDGF-BB Proteins and Enzymes
• PDGF-BB ELISAs