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Recombinant Human RDH12 His Protein

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SDS-Page: Recombinant Human RDH12 His Protein [NBP1-78870] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
SDS-Page: Recombinant Human RDH12 His Protein [NBP1-78870] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.25 mg/ml

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Recombinant Human RDH12 His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 39-316 of Human RDH12

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMGKVVV ITGANTGIGK ETARELASRG ARVYIACRDV LKGESAASEI RVDTKNSQVL VRKLDLSDTK SIRAFAEGFL AEEKQLHILI NNAGVMMCPY SKTADGFETH LGVNHLGHFL LTYLLLERLK VSAPARVVNV SSVAHHIGKI PFHDLQSEKR YSRGFAYCHS KLANVLFTRE LAKRLQGTGV TTYAVHPGVV RSELVRHSSL LCLLWRLFSP FVKTAREGAQ TSLHCALAEG LEPLSGKYFS DCKRTWVSPR ARNNKTAERL WNVSCELLGI RWE
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
RDH12
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
33.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 2 mM DTT, 40% glycerol, 0.2 M NaCl
Preservative
No Preservative
Concentration
0.25 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human RDH12 His Protein

  • All-trans and 9-cis retinol dehydrogenase
  • EC 1.1.1
  • EC 1.1.1.-
  • EC 1.1.1.100
  • FLJ30273
  • LCA13retinol dehydrogenase 12, all-trans and 9-cis
  • LCA3
  • retinol dehydrogenase 12 (all-trans and 9-cis)
  • retinol dehydrogenase 12 (all-trans/9-cis/11-cis)
  • retinol dehydrogenase 12
  • SDR7C2
  • short chain dehydrogenase/reductase family 7C, member 2

Background

Retinol dehydrogenase 12, also known as RDH12, belongs to the short-chain dehydrogenases /reductases (SDR) family. RDH12 is expressed, mostly in eye, kidney, brain, skeletal muscle and stomach. It is an NADPH-dependent retinal reductase whose highest activity is toward 9-cis and all-trans-retinol. RDH12 also plays a role in the metabolism of short-chain aldehydes but does not exhibit steroid dehydrogenase activity. Recombinant human RDH12 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol RDH12