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Recombinant Human Rad1 His Protein

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3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

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Recombinant Human Rad1 His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-282 of Human Rad1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPLLTQQIQD EDDQYSLVAS LDNVRNLSTI LKAIHFREHA TCFATKNGIK VTVENAKCVQ ANAFIQAGIF QEFKVQEESV TFRINLTVLL DCLSIFGSSP MPGTLTALRM CYQGYGYPLM LFLEEGGVVT VCKINTQEPE ETLDFDFCST NVINKIILQS EGLREAFSEL DMTSEVLQIT MSPDKPYFRL STFGNAGSSH LDYPKDSDLM EAFHCNQTQV NRYKISLLKP STKALVLSCK VSIRTDNRGF LSLQYMIRNE DGQICFVEYY CCPDEEVPES ES
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
RAD1
Purity
>85%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
33.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0), 0.2 M Nacl, 1 mM DTT, 10% glycerol
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>85%, by SDS-PAGE

Alternate Names for Recombinant Human Rad1 His Protein

  • cell cycle checkpoint protein Hrad1
  • checkpoint control protein HRAD1
  • DNA repair exonuclease rad1 homolog
  • DNA repair exonuclease REC1
  • EC 3.1.11.2
  • exonuclease homolog RAD1
  • HRAD1
  • RAD1 (S. pombe) homolog
  • RAD1 homolog (S. pombe)
  • Rad1-like DNA damage checkpoint protein
  • REC1cell cycle checkpoint protein RAD1

Background

RAD1 is a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. This complex also contains the Rad9 and Hus1 proteins and is believed to be involved in cellular responses to DNA damage, possibly by associating with Rad17 and several components of the PCNA-loading heteropentamer, replication factor C. Recombinant human RAD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol RAD1