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Rabbit TNF-alpha DuoSet ELISA, 15 Plate

 (19 Publications)
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Summary
Reactivity RbSpecies Glossary
Applications ELISA
Conjugate
Biotin

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Rabbit TNF-alpha DuoSet ELISA, 15 Plate Summary

Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
TNF

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DY5670.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rabbit TNF-alpha DuoSet ELISA, 15 Plate

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • TNFA
  • TNF-A
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFG1F
  • TNFSF1A
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha

Background

Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. Human TNF-α consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-α shares 97% aa sequence identity with rhesus and 71% - 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness.

Cleavage of membrane bound TNF-α by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-α. TNF-α trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers. TNF-α regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF-α is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.

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Rabbit TNF-alpha
DuoSet ELISA, 15 Plat
DY5670
Rabbit TNF-alpha DuoSet ELISA, 15 Plate
Species: Rb
Applications: ELISA

Publications for TNF-alpha (DY5670)(19)

We have publications tested in 2 confirmed species: Rat, Rabbit.


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Rat
(1)
Rabbit
(18)
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Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using DY5670 Applications Species
De Vera, CJ;Jacob, J;Sarva, K;Christudas, S;Emerine, RL;Florence, JM;Akiode, O;Gorthy, TV;Tucker, TA;Singh, KP;Azghani, AO;Komissarov, AA;Florova, G;Idell, S; Intrapleural Fibrinolytic Interventions for Retained Hemothoraces in Rabbits International journal of molecular sciences 2024-08-12 [PMID: 39201465] (Rabbit) Rabbit
De Vera, CJ;Emerine, RL;Girard, RA;Sarva, K;Jacob, J;Azghani, AO;Florence, JM;Cook, A;Norwood, S;Singh, KP;Komissarov, AA;Florova, G;Idell, S; A Novel Rabbit Model of Retained Hemothorax with Pleural Organization International journal of molecular sciences 2023-12-29 [PMID: 38203639] (Rabbit) Rabbit
Florova, G;De Vera, CJ;Emerine, RL;Girard, RA;Azghani, AO;Sarva, K;Jacob, J;Morris, DE;Chamiso, M;Idell, S;Komissarov, AA; Targeting the PAI-1 Mechanism with a Small Peptide Increases the Efficacy of Alteplase in a Rabbit Model of Chronic Empyema Pharmaceutics 2023-05-14 [PMID: 37242740] (Rabbit) Rabbit
M Yao, Z Wei, J Li, Z Guo, Z Yan, X Sun, Q Yu, X Wu, C Yu, F Yao, S Feng, H Zhang, J Li Microgel reinforced zwitterionic hydrogel coating for blood-contacting biomedical devices Nature Communications, 2022-09-12;13(1):5339. 2022-09-12 [PMID: 36096894] (Rabbit) Rabbit
HJ Go, BJ Park, HS Ahn, SH Han, DH Kim, EL Lyoo, DY Kim, JH Kim, JB Lee, SY Park, CS Song, SW Lee, YK Choi, IS Choi Immunization with Virus-Like Particle Vaccine Protects Rabbits against Hepatitis E-3 Virus Infection Viruses, 2022-06-29;14(7):. 2022-06-29 [PMID: 35891413] (Rat) Rat
W Wang, D Pan, W Fu, X Ye, J Han, L Yang, J Jia, J Liu, R Zhu, Y Zhang, C Liu, J Ye, A Selariu, Y Que, Q Zhao, T Wu, Y Li, J Zhang, T Cheng, H Zhu, N Xia Development of a skin- and neuro-attenuated live vaccine for varicella Nature Communications, 2022-02-11;13(1):824. 2022-02-11 [PMID: 35149692] (Rabbit) Rabbit
C Zhang, X Wang, S Sun, Y Fu, Y Wu, S Zhao, X Fan, E Liu Fat-1 expression alleviates atherosclerosis in transgenic rabbits Journal of Cellular and Molecular Medicine, 2022-01-18;0(0):. 2022-01-18 [PMID: 35040258] (Rabbit) Rabbit
J Du, Z Kang, L Huang, F Zhou, X Feng, J Huang Protective effects of Hirudin against compartment syndrome in rabbits through the activation of Nrf2/HO-1 Injury, 2021-11-23;0(0):. 2021-11-23 [PMID: 34872701] (Rabbit) Rabbit
G Florova, RA Girard, AO Azghani, K Sarva, A Buchanan, S Karandasho, CJ DeVera, D Morris, M Chamiso, K Koenig, DB Cines, S Idell, AA Komissarov Precision targeting of the plasminogen activator inhibitor-1 mechanism increases efficacy of fibrinolytic therapy in empyema Physiological Reports, 2021-05-01;9(9):e14861. 2021-05-01 [PMID: 33991465] (Rabbit) Rabbit
GYC Cheung, JS Bae, R Liu, RL Hunt, Y Zheng, M Otto Bacterial virulence plays a crucial role in MRSA sepsis PloS Pathogens, 2021-02-25;17(2):e1009369. 2021-02-25 [PMID: 33630954] (Rabbit) Rabbit
Show All 19 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TNF-alpha (DY5670). (Showing 1 - 4 of 4 FAQs).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

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Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ...  Read full blog post.
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By Hunter MartinezWhat is Immunometabolism?It is well established that abnormal metabolic environments can be a risk factor for disease development. One characteristic example is the role of dyslipidemia (high lev...  Read full blog post.

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By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.
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You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
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Bioinformatics

Gene Symbol TNF