![Western Blot: PTK9 Antibody (OTI5C12) - Azide and BSA Free [NBP2-73710] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TWF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TWF1.](http://images.novusbio.com/fullsize/PTK9-Antibody-OTI5C12-Azide-and-BSA-Free-Western-Blot-NBP2-73710-img0002.jpg "Western Blot: PTK9 Antibody (OTI5C12) - Azide and BSA Free [NBP2-73710] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TWF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TWF1.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC, KO |
| Clone | OTI5C12 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 140-384 of human TWF1(NP_002813) produced in E.coli. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TWF1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 43.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
![Western Blot RFC1 Antibody [Unconjugated]](https://images.novusbio.com/images2/RFC1_AF6457_Western_Blot_9874.jpg)
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Secondary Antibodies |
Isotype Controls |
Research Areas for PTK9 Antibody (NBP2-73710)Find related products by research area.
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| Gene Symbol | TWF1 |
![Immunohistochemistry: PTK9 Antibody (OTI5C12) - Azide and BSA Free [NBP2-73710] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TWF1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PTK9-Antibody-OTI5C12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73710-img0004.jpg "Immunohistochemistry: PTK9 Antibody (OTI5C12) - Azide and BSA Free [NBP2-73710] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TWF1 mouse monoclonal antibody.")
![Western Blot: PTK9 Antibody (OTI5C12) - Azide and BSA Free [NBP2-73710] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and TWF1-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-TWF1 monoclonal antibody. Then the blotted membrane was stripped and reprobed with an](http://images.novusbio.com/fullsize/PTK9-Antibody-OTI5C12-Azide-and-BSA-Free-Western-Blot-NBP2-73710-img0001.jpg "Western Blot: PTK9 Antibody (OTI5C12) - Azide and BSA Free [NBP2-73710] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and TWF1-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-TWF1 monoclonal antibody. Then the blotted membrane was stripped and reprobed with an")
![Immunohistochemistry: PTK9 Antibody (OTI5C12) - Azide and BSA Free [NBP2-73710] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TWF1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PTK9-Antibody-OTI5C12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73710-img0003.jpg "Immunohistochemistry: PTK9 Antibody (OTI5C12) - Azide and BSA Free [NBP2-73710] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TWF1 mouse monoclonal antibody.")
![Immunohistochemistry: PTK9 Antibody (OTI5C12) - Azide and BSA Free [NBP2-73710] - Staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TWF1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PTK9-Antibody-OTI5C12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73710-img0005.jpg "Immunohistochemistry: PTK9 Antibody (OTI5C12) - Azide and BSA Free [NBP2-73710] - Staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TWF1 mouse monoclonal antibody.")
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