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PPOX Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: PPOX Antibody [NBP1-80885] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & mitochondria.
Immunohistochemistry: PPOX Antibody [NBP1-80885] - Staining of human adrenal gland shows strong nuclear and cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PPOX Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ETHLTSRGVSVLRGQPVCGLSLQAEGRWKVSLRDSSLEADHVISAIPASVLSELLPAEAAPLARALSAITAVSVAVVNLQYQGAH
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPOX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PPOX Protein (NBP1-80885PEP)
Publications
Read Publications using NBP1-80885.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PPOX Antibody

  • EC 1.3.3.4
  • MGC8485
  • PPOVP
  • protoporphyrinogen oxidase
  • V290M
  • variegate porphyria

Background

PPOX encodes the penultimate enzyme of heme biosynthesis, which catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. Mutations in this gene cause variegate porphyria, an autosomal dominant disorder of heme metabolism resulting from a deficiency in protoporphyrinogen oxidase, an enzyme located on the inner mitochondrial membrane. Alternatively spliced transcript variants encoding the same protein have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PPOX Antibody (NBP1-80885)(2)


Showing Publications 1 - 2 of 2.
Publications using NBP1-80885 Applications Species
vorgelegt v, Wen W Expression and function of blood receptors ROR?, REVERBA and BMAL1 in human colorectal cancer Thesis 2022-01-01
Weimar, HV, HV Investigating the Impacts of Maternal Cannabis Use on Developing Offspring in Rats Thesis 2022-01-01

Reviews for PPOX Antibody (NBP1-80885) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for PPOX Antibody (NBP1-80885) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on PPOX

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Bioinformatics

Gene Symbol PPOX
Entrez
Uniprot