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Human POT1 ELISA Kit (Colorimetric)

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ELISA: Human POT1 ELISA Kit (Colorimetric) [NBP3-39027] - Standard Curve Reference
ELISA: Human POT1 ELISA Kit (Colorimetric) [NBP3-39027] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Tissue homogenates, cell lysates and other biological fluids
Standard Curve Range
0.312 - 20 ng/mL (example only; lot dependent)
Sensitivity
0.114 ng/mL (example only; lot dependent)

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Human POT1 ELISA Kit (Colorimetric) Summary

Description
Assay Length: 4.5 hours
Standard Curve Range
0.312 - 20 ng/mL (example only; lot dependent)
Sensitivity
0.114 ng/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
POT1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Reagent A
  2. Detection Reagent B
  3. Diluent Buffer
  4. Instruction manual
  5. Plate sealer for 96 wells
  6. Pre-coated 96T strip plate
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human POT1 ELISA Kit (Colorimetric)

  • DKFZp586D211
  • HPOT1
  • POT1 protection of telomeres 1 homolog
  • POT1
  • POT1-like telomere end-binding protein
  • protection of telomeres 1 homolog (S. pombe)
  • protection of telomeres protein 1

Background

POT1 (protection of telomeres 1), a telomere regulator, is a single-stranded telomeric DNA-binding protein that controls telomerase-mediated telomere elongation. Telomere maintenance is required for protection against changes in telomere length, which has been implicated in ageing and certain cancers. Specifically, this protein functions as a member of a multi-protein complex that binds to the TTAGGG repeats of telomeres, regulating telomere length and protecting chromosome ends from illegitimate recombination, catastrophic chromosome instability, and abnormal chromosome segregation. Excessive transcriptional expression of POT1 has been associated with stomach cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Telomeres are a region of repeat nucleotide sequences located at the end of chromosomes to protect our DNA from becoming damaged via end-to-end fusion.  TERF2, or telomeric-repeat binding factor 2, is important for telomere integrity and aids in th...  Read full blog post.
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Bioinformatics

Gene Symbol POT1