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PLD1 Antibody (2F3)

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Western Blot: PLD1 Antibody (2F3) [H00005337-M01] - Analysis of PLD1 expression in transfected 293T cell line by PLD1 monoclonal antibody (M01), clone 2F3.Lane 1: PLD1 transfected lysate(124.2 KDa).Lane 2: Non-transfected lysate.
Western Blot: PLD1 Antibody (2F3) [H00005337-M01] - Analysis of PLD1 expression in transfected 293T cell line by PLD1 monoclonal antibody (M01), clone 2F3.Lane 1: PLD1 transfected lysate(124.2 KDa).Lane 2: ...read more
Sandwich ELISA: PLD1 Antibody (2F3) [H00005337-M01] - Detection limit for recombinant GST tagged PLD1 is 0.03 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
2F3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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PLD1 Antibody (2F3) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
PLD1 (NP_002653, 965 a.a. ~ 1074 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GYLDDPSEDIQDPVSDKFFKEVWVSTAARNATIYDKVFRCLPNDEVHNLIQLRDFINKPVLAKEDPIRAEEELKKIRGFLVQFPFYFLSEESLLPSVGTKEAIVPMEVWT
Specificity
PLD1 - phospholipase D1, phophatidylcholine-specific
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PLD1
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.
Publications
Read Publication using H00005337-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PLD1 Antibody (2F3)

  • Choline phosphatase 1
  • EC 3.1.4.4
  • hPLD1
  • Phosphatidylcholine-hydrolyzing phospholipase D1
  • phospholipase D1
  • phospholipase D1, phophatidylcholine-specific
  • phospholipase D1, phosphatidylcholine-specific
  • PLD 1
  • PLD1

Background

Phosphatidylcholine (PC)-specific phospholipases D (PLDs; EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. A range of agonists acting through G protein-coupled receptors and receptor tyrosine kinases stimulate this hydrolysis. PC-specific PLD activity has been implicated in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis (Hammond et al., 1995 [PubMed 8530346]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PLD1
Entrez
OMIM
Uniprot