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PLA2G3 Antibody

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Western Blot: PLA2G3 Antibody [NBP1-83367] - Analysis in control (vector only transfected HEK293T lysate) and PLA2G3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T ...read more
Immunohistochemistry-Paraffin: PLA2G3 Antibody [NBP1-83367] - Staining of human skin shows moderate membranous and cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: PLA2G3 Antibody [NBP1-83367] - Staining of human lung shows strong membranous and cytoplasmic positivity in macrophages.
Immunohistochemistry-Paraffin: PLA2G3 Antibody [NBP1-83367] - Staining of human endometrium shows weak membranous and cytoplasmic positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: PLA2G3 Antibody [NBP1-83367] - Staining of human skeletal muscle shows no membranous and cytoplasmic positivity in myocytes as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PLA2G3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SPPEVTNMLWELLGTTCFKLAPPLDCVEGKNCSRDPRAIRVSARHLRRLQQRRHQLQDKGTDERQPWPSEPLRGPMSFYNQCLQLTQAARRPDRQQKSWSQ
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLA2G3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
PLA2G3 Protein (NBP1-83367PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PLA2G3 Antibody

  • EC 3.1.1.4
  • GIII SPLA2
  • GIII-SPLA2
  • group 3 secretory phospholipase A2
  • group III secreted phospholipase A2
  • Group III secretory phospholipase A2
  • Phosphatidylcholine 2-acylhydrolase 3
  • phosphatidylcholine 2-acylhydrolase GIII
  • phospholipase A2, group III
  • PLA2G3
  • SPLA2III
  • SPLA2-III

Background

PLA2G3 belongs to the family of secreted phospholipase A2 (sPLA2; EC 3.1.1.4) proteins. These Ca(2+)-dependent lipolytic enzymes have a conserved Ca(2+)-binding loop and a his-asp dyad in the catalytic site (Murakami et al., 2003 [PubMed 12522102]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PLA2G3