| Reactivity | HuSpecies Glossary |
| Applications | ICC/IF |
| Clone | 585509 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | E. coli-derived recombinant human PIWIL1/HIWI Arg82-Thr290 Accession # NP_004755 |
| Specificity | Detects human PIWIL1/HIWI in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) PIWIL2 or rhPIWIL4 is observed. |
| Source | N/A |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PIWIL1 |
| Purity Statement | Protein A or G purified from hybridoma culture supernatant |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Sterile PBS to a final concentration of 0.5 mg/mL. |
HIWI (human homolog of drosophila piwi), also called PIWIL1 (piwi-like 1) is an 861 amino acid, 99 kDa (predicted) cytoplasmic protein of the PIWI subgroup of the Argonaute family. PIWI domains bind a class of ~29-30 nucleotide RNAs termed piRNAs (piwi domain-binding RNAs). PIWIL1 orthologs are expressed with piRNAs in germ cells and are essential for spermatogenesis in mice. CD34+ hematopoietic progenitor cell PIWIL1 is downregulated upon differentiation. When overexpressed in leukemic cell lines, PIWIL1 inhibits growth and induces apoptosis. Human PIWIL1 shares 95% amino acid sequence identity with mouse and rat PIWIL1.
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot VASA Antibody [Unconjugated]](https://images.novusbio.com/images2/VASA_AF2030_Western_Blot_16711.jpg)
![Immunohistochemistry VASA Antibody [Unconjugated]](https://images.novusbio.com/images2/VASA_AF2030_Immunohistochemistry_7144.jpg)
![Immunohistochemistry VASA Antibody [Unconjugated]](https://images.novusbio.com/images/af2030_human-vasa-affinity-purified-polyclonal-ab-4120241239493.jpg)
![Immunocytochemistry Goosecoid Antibody [Unconjugated]](https://images.novusbio.com/images2/Goosecoid_AF4086_Immunocytochemistry__Immunofluorescence_20128.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")