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p57 Kip2 Antibody

Images

 
Western Blot: p57 Kip2 Antibody [NBP3-27877] - Western blot analysis of p57 Kip2 in 293 cell lysate with p57 Kip2 antibody at (A) 1 and (B) 2 u/mL.
Immunocytochemistry/ Immunofluorescence: p57 Kip2 Antibody [NBP3-27877] - Immunofluorescence of p57 Kip2 in 293 cells with p57 Kip2 antibody at 20 u/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

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p57 Kip2 Antibody Summary

Immunogen
p57 Kip2 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human p57 Kip2. The immunogen is located within amino acids 40 - 90 of p57 Kip2.
Specificity
At least three isoforms of p57 Kip2 are known to exist; this antibody will detect all three isoforms. Despite its predicted molecular weight, p57 Kip2 often migrates at 57kDa in SDS-PAGE. CDKN1C antibody is predicted to not cross react with other CIP/KIP proteins.
Clonality
Polyclonal
Host
Rabbit
Gene
CDKN1C
Purity
Peptide affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Western Blot 1 - 2 ug/mL

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for p57 Kip2 Antibody

  • Beckwith-Wiedemann syndrome
  • BWS
  • cyclin-dependent kinase inhibitor 1C (p57, Kip2)
  • cyclin-dependent kinase inhibitor 1C
  • Cyclin-dependent kinase inhibitor p57
  • KIP2BWCR
  • p57
  • p57Kip2
  • WBS

Background

p57kip2 is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CDKN1C
Uniprot