![Western Blot: ODF2 [p Ser796] Antibody [NB110-85525] - Western Blot of ODF2 [p Ser796] antibody. Lane 1: MW.Lane 2: Human Semen Lysate.Lane 3: MCF7 WCL. Lane 4: Molt 4 WCL. Lane 5: HeLa WCL. Load: 10ug per lane. Blocking:. Primary Antibody: Anti-ODF2 pS796 at 1ug/mL overnight at 2-8C.Secondary Antibody: Goat anti-Rabbit HRP 1:40,000 diluted in for 30 minutes at RT.Expect: 93kDa.](http://images.novusbio.com/fullsize/ODF2-[p-Ser796]-Antibody-Western-Blot-NB110-85525-img0005.jpg "Western Blot: ODF2 [p Ser796] Antibody [NB110-85525] - Western Blot of ODF2 [p Ser796] antibody. Lane 1: MW.Lane 2: Human Semen Lysate.Lane 3: MCF7 WCL. Lane 4: Molt 4 WCL. Lane 5: HeLa WCL. Load: 10ug per lane. Blocking:. Primary Antibody: Anti-ODF2 pS796 at 1ug/mL overnight at 2-8C.Secondary Antibody: Goat anti-Rabbit HRP 1:40,000 diluted in for 30 minutes at RT.Expect: 93kDa.")
| Reactivity | Hu, Pm, RMSpecies Glossary |
| Applications | WB, DB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against nonphospho-peptide Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | This affinity purified ODF2 [p Ser796] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near S796 of human ODF2. (Uniprot: Q5BJF6) |
| Modification | p Ser796 |
| Specificity | This antibody is specific for human ODF2 protein phosphorylated at S796. A BLAST analysis was used to suggest cross-reactivity with ODF2 from human, chimpanzee and macaque based on 100% homology with the immunizing sequence. Cross-reactivity with ODF2from other sources has not been determined. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ODF2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 93 kDa in size corresponding to phosphorylated Cenexin-1 by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with human Cenexin-1 pS796 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. |
| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Immunogen affinity purified |
![ODF2 [p Ser796] Antibody](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "ODF2 [p Ser796] Antibody")
Secondary Antibodies |
Isotype Controls |
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![ELISA: ODF2 [p Ser796] Antibody [NB110-85525] - ELISA of ODF2 [p Ser796] antibody. Antigen: BSA conjugates of ODF2 pS796 (purple) and S796 (blue).Coating amount: 0.1 ug per well. Primary antibody: ODF2 at 5 ug/mL. Secondary antibody: Peroxidase goat anti-rabbit secondary antibody at 1:8,500. Substrate: TMB.](http://images.novusbio.com/fullsize/ODF2-[p-Ser796]-Antibody-ELISA-NB110-85525-img0006.jpg "ELISA: ODF2 [p Ser796] Antibody [NB110-85525] - ELISA of ODF2 [p Ser796] antibody. Antigen: BSA conjugates of ODF2 pS796 (purple) and S796 (blue).Coating amount: 0.1 ug per well. Primary antibody: ODF2 at 5 ug/mL. Secondary antibody: Peroxidase goat anti-rabbit secondary antibody at 1:8,500. Substrate: TMB.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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