![Western Blot: OCRL Antibody (S166A-26) [NBP2-42193] - analysis of Monkey COS cells transfected with GFP-tagged OCRL showing detection of 100 kDa INPP5F protein using Mouse Anti-INPP5F Monoclonal Antibody, Clone S166A-26 . Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged OCRL. Load: 15 ug . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-INPP5F Monoclonal Antibody at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 100 kDa.](http://images.novusbio.com/fullsize/OCRL-Antibody-S166A-26-Western-Blot-NBP2-42193-img0001.jpg "Western Blot: OCRL Antibody (S166A-26) [NBP2-42193] - analysis of Monkey COS cells transfected with GFP-tagged OCRL showing detection of 100 kDa INPP5F protein using Mouse Anti-INPP5F Monoclonal Antibody, Clone S166A-26 . Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged OCRL. Load: 15 ug . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-INPP5F Monoclonal Antibody at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 100 kDa.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | S166A-26 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Fusion protein amino acids 1-901 (full-length) of human INPP5F. Rat: 93% identity (845/904 amino acids identical). Mouse: 91% identity (824/901 amino acids identical) ~50% identity with INPP5b. |
| Localization | Cytoplasmic Vesicle , Endosome , Endosome Membrane , Membrane , Golgi Apparatus |
| Specificity | Approx 50% identity with INPP5b.Detects approx 100kDa. Cross-reacts with INPP5b. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | OCRL |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | 1 ug/ml of INPP5F Antibody was sufficient for detection of INPP5F in 20 ug of COS cells transiently transformed with GFP-tagged OCLR lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 50% Glycerol |
| Preservative | 0.1% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: OCRL Antibody (S166A-26) [NBP2-42193] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-INPP5F Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasmic Vesicle, Endosome, Endosome Membrane, Membrane, Golgi Apparatus, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/OCRL-Antibody-S166A-26-Immunocytochemistry-Immunofluorescence-NBP2-42193-img0002.jpg "Immunocytochemistry/Immunofluorescence: OCRL Antibody (S166A-26) [NBP2-42193] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-INPP5F Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasmic Vesicle, Endosome, Endosome Membrane, Membrane, Golgi Apparatus, Nucleus. Magnification: 60X.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")