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Human MYH4 ELISA Kit (Colorimetric)

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ELISA: Human MYH4 ELISA Kit (Colorimetric) [NBP3-39542] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Standard Curve Range
0.312 - 20 ng/mL (example only; lot dependent)
Sensitivity
0.127 ng/mL (example only; lot dependent)

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Human MYH4 ELISA Kit (Colorimetric) Summary

Description
Assay Length: 4.5 hours
Standard Curve Range
0.312 - 20 ng/mL (example only; lot dependent)
Sensitivity
0.127 ng/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
MYH4

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Reagent A
  2. Detection Reagent B
  3. Diluent Buffer
  4. Instruction manual
  5. Plate sealer for 96 wells
  6. Pre-coated 96T strip plate
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human MYH4 ELISA Kit (Colorimetric)

  • MYH4
  • MyHC-2b
  • MyHC-IIb
  • myosin heavy chain 4
  • myosin heavy chain IIb
  • myosin heavy chain, skeletal muscle, fetal
  • myosin, heavy chain 4, skeletal muscle
  • myosin, heavy polypeptide 4, skeletal muscle
  • myosin-4

Background

MYH4, also known as Myosin-4, is a 1939 amino acid protein that is 223 kDa, predominantly found in myocytes mediates plus-ended movement along microfilaments and involved in muscle contraction through cyclic interactions with actin-rich thin filaments, creating a contractile force. It is regulated by phosphorylation via myosin light chain kinase (MLCK) and by intracellular Ca2+ concentrations. Current research is being performed on several diseases and disorders including congestive heart failure, yaws, myocarditis, hermaphroditism, liver disease, pharyngitis, and alcoholism. This protein is involved in cell adhesion integrin-mediated cell adhesion and migration, cell adhesion tight junctions, cytoskeleton remodeling regulation of actin cytoskeleton by Rho GTPases, immune response CCR3 signaling in eosinophils, development MAG-dependent inhibition of neurite outgrowth, RhoA, antioxidant action of vitamin-C, transendothelial migration of leukocytes, actin nucleation by ARP-WASP complex, epithelial adherens junctions, inhibitory action of lipoxins on neutrophil migration, membrane trafficking, translocation of GLUT4 to the plasma membrane, translocation of GLUT4 vesicle and docking at the plasma membrane, and Viral myocarditis pathways forming interactions with ESR2, NTHL1, S100A4, USP15, C12orf44, CNBP, ACTB, and over 30 other proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol MYH4