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Human Metallothionein-1E ELISA Kit (Colorimetric)

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ELISA: Human Metallothionein-1E ELISA Kit (Colorimetric) [NBP3-06947] - Samples were spiked with high concentrations of Human Metallothionein-1E and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human Metallothionein-1E ELISA Kit (Colorimetric) [NBP3-06947] - Samples were spiked with high concentrations of Human Metallothionein-1E and diluted with Reference Standard & Sample Diluent to produce samples ...read more
ELISA: Human Metallothionein-1E ELISA Kit (Colorimetric) [NBP3-06947] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Cell supernatant
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL

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Human Metallothionein-1E ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human Metallothionein-1E in samples. No significant cross-reactivity or interference between Human Metallothionein-1E and analogues was observed.
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
5.28
Intra-Assay
4.61
Spike Recovery
89-108 %
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
MT1E

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Metallothionein-1E ELISA Kit (Colorimetric)

  • metallothionein 1E (functional)
  • metallothionein 1E
  • metallothionein D
  • metallothionein-1E
  • metallothionein-IE
  • MT1
  • MT-1E
  • MTD
  • MT-IE

Background

Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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  Human Metallothionein-1E ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol MT1E